Anti-PTGR3/ZADH2 Polyclonal Antibody for WB, IHC-P, ELISA - Q8N4Q0

Core Product Specifications and Parameters

Biological Background: PTGR3/ZADH2 Function and Localization

• PTGR3, also known as ZADH2 or PRG-3, is a member of the zinc-binding alcohol dehydrogenase family and functions as a prostaglandin reductase.
• It catalyzes the 13-reduction of 15-keto-prostaglandins, with the highest efficiency for 15-keto-PGE2-alpha, playing a critical role in prostanoid metabolism.
• Overexpression of PTGR3 inhibits adipocyte differentiation by repressing the transcriptional activity of PPARG.
• The protein localizes to peroxisomes, consistent with its involvement in lipid metabolism.
• PTGR3 is subject to post-translational modifications such as acetylation and phosphorylation, which may regulate its activity.
• The gene undergoes alternative splicing, leading to multiple protein isoforms.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 33–371 of human PTGR3 (NP_787103.1), encompassing nearly the full-length protein; this design should confer high specificity and sensitivity in detection assays.
• For Western blotting, a band at approximately 40 kDa is expected; consider using whole-cell lysates from human, mouse, or rat tissues/cell lines known to express PTGR3.
• In immunohistochemistry (IHC-P), optimize antigen retrieval and antibody dilution; validate in peroxisome-rich tissues such as liver or kidney.
• For ELISA, this antibody can be used as a capture or detection reagent; pair with a suitable monoclonal antibody if developing a sandwich assay for PTGR3 quantification.
• Since the antibody is a rabbit polyclonal, it may exhibit lot-to-lot variability; include appropriate positive and negative controls in each experiment.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with reliable access to high-quality IVD raw materials, including validated antibodies for prostaglandin metabolism and metabolic disease research. Beyond this PTGR3 polyclonal antibody, we offer a comprehensive portfolio of monoclonal antibodies, antibody pairs, and bulk ancillary reagents to support your assay development from concept to clinic. Leverage our sourcing expertise to obtain rare or custom targets efficiently.