Anti-PTGIR Polyclonal Antibody for WB, IHC-P, ELISA - P43119

Core Product Specifications and Parameters

Biological Background: PTGIR Function and Localization

• PTGIR, also known as prostacyclin receptor, prostaglandin I2 receptor, PGI receptor, or prostanoid IP receptor, is a member of the G protein-coupled receptor (GPCR) superfamily.
• It functions as the receptor for prostacyclin (prostaglandin I2 or PGI2), a potent vasodilator and inhibitor of platelet aggregation.
• Upon ligand binding, PTGIR primarily couples to G(s) proteins, leading to the activation of adenylate cyclase and increased intracellular cAMP levels.
• The receptor is localized to the cell membrane, consistent with its role as a transmembrane signal transducer.
• PTGIR undergoes several post-translational modifications, including glycosylation, palmitoylation (lipoprotein), methylation, and prenylation, which are characteristic for GPCR maturation and trafficking.
• Its structure includes seven transmembrane helices, a disulfide bond, and is subject to proteomics identification as a reference proteome component.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 207–386, an intracellular region of PTGIR; this design may favor detection of total receptor forms in denaturing and native assays.
• For Western blot, PTGIR has a predicted molecular weight of approximately 41 kDa; a band near this size is expected, though post-translational modifications may cause slight shifts. Validate with appropriate controls in your sample system.
• In IHC-P applications, consider optimization of antigen retrieval and antibody dilution, as paraffin-embedded tissues may present epitope accessibility variations.
• While the antibody is listed for ELISA, direct antigen-coating formats using recombinant PTGIR fragments may be suitable; indirect detection schemes should be validated with the specific assay design.
• Cross-reactivity with mouse and rat PTGIR suggests utility in rodent models; however, confirm sequence homology between the immunogen and orthologous proteins for your specific application.

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