Anti-PTGFR Rabbit Polyclonal Antibody for WB - P43088

Core Product Specifications and Parameters

Biological Background: PTGFR Function and Localization

• The PTGFR gene encodes the prostaglandin F2-alpha receptor (also known as prostanoid FP receptor), a G protein-coupled receptor that mediates cellular responses to PGF2-alpha.
• Upon PGF2-alpha binding, the receptor activates a phosphatidylinositol-calcium second messenger system via G proteins, triggering downstream signaling cascades (By similarity).
• Isoforms 2 through 7 arise from alternative splicing; while they do not bind PGF2-alpha independently, they may modulate signaling by forming variant heteromeric complexes with isoform 1.
• A heterodimer between isoform 1 and isoform 5 is proposed to function as a receptor for prostamides, including the synthetic analog bimatoprost.
• The receptor is localized at the cell membrane and contains seven transmembrane domains, consistent with its GPCR classification.
• Tissue expression profiling indicates high specificity in the eye, where the receptor is involved in physiological processes such as regulating intraocular pressure.
• The protein undergoes post-translational modifications including glycosylation and disulfide bond formation, typical for membrane receptors, and is classified under keywords like alternative splicing, glycoprotein, and transducer.

Experimental Guidance and Technical Tips

• The immunogen was designed from a synthetic peptide spanning amino acids 50–150, corresponding to an extracellular/transmembrane region of human PTGFR; this may enable detection of multiple isoforms.
• For Western blotting, a band near 40 kDa is expected for isoform 1; consider using membrane-enriched protein extracts to enhance signal.
• The antibody’s cross-reactivity with mouse and rat suggests conserved epitopes; validate its performance in relevant tissue or cell models (e.g., eye-derived samples).
• In ELISA, the peptide immunogen can serve as a positive control to assess binding. Note that detection of native, glycosylated receptor may require optimized coating conditions.
• Given the existence of splice variants, confirm specificity by siRNA knockdown or overexpression experiments if isoform discrimination is needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic developers and research labs with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and rare-target sourcing. This anti-PTGFR polyclonal antibody supports WB and ELISA applications across human, mouse, and rat samples, addressing GPCR-related research and ocular biology studies. Whether you need ready-to-use antibodies or customized raw-material procurement, CamelBio serves as your integrated sourcing partner from concept to clinic.