Anti-PTGES2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9H7Z7

Core Product Specifications and Parameters

Biological Background: PTGES2 Function and Localization

• PTGES2, also known as mPGES-2, GBF1, or C9orf15, is a prostaglandin E synthase encoded by the PTGES2 gene (Gene ID: 80142).
• It functions as an isomerase that catalyzes in vitro conversion of prostaglandin H2 (PGH2) to prostaglandin E2 (PGE2), though its in vivo role may involve degradation of PGH2 to HHT and MDA (By similarity). Related references: PMID:12804604, PMID:17585783, PMID:18198127
• PTGES2 is a membrane-associated protein localized primarily to the Golgi apparatus membrane and perinuclear cytoplasmic regions.
• It is widely expressed, with high levels in heart (atria, ventricles, septum), brain (cerebellum, occipital, frontal, parietal lobes), lymph nodes, skeletal muscle, kidney, and trachea; notably overexpressed in colorectal cancer.
• The protein is classified as a phosphoprotein and contains transmembrane helices; it is involved in lipid biosynthesis, specifically fatty acid and prostaglandin metabolism.
• Key alternative names include Prostaglandin-H(2) E-isomerase, microsomal prostaglandin E synthase 2, and GBF1.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 88–377 of human PTGES2, covering a large portion of the protein; this design may enable detection of both full-length and some truncated isoforms.
• For Western blotting, a band around 42 kDa is expected; validate with lysates from PTGES2-expressing tissues or cell lines, such as colorectal cancer samples.
• For immunohistochemistry and immunofluorescence, consider testing antigen retrieval conditions and use tissues with high endogenous PTGES2 expression (e.g., cardiac muscle, brain).
• The antibody is reported to cross-react with mouse and rat PTGES2; therefore, it may be suitable for studies in rodent models, though independent validation is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering a broad portfolio that includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti-PTGES2 polyclonal antibody supports research in prostaglandin metabolism and related disorders; contact us for tailored sourcing and technical consultation.