Anti-PTEN Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P60484

Core Product Specifications and Parameters

Biological Background: PTEN Function and Localization

• PTEN is a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine-, and threonine-phosphorylated proteins (PubMed:9187108, PubMed:9256433, PubMed:9616126). Related references: PMID:9187108 PMID:9256433 PMID:9616126
• It functions as a lipid phosphatase, removing the D3 phosphate from PtdIns(3,4,5)P3, PtdIns(3,4)P2, and PtdIns3P, thereby antagonizing the PI3K-AKT/PKB signaling pathway (PubMed:16824732, PubMed:26504226, PubMed:9593664). Related references: PMID:16824732 PMID:26504226 PMID:9593664
• PTEN also acts as a cytosolic inositol 3-phosphatase, hydrolyzing Ins(1,3,4,5,6)P5 and possibly Ins(1,3,4,5)P4 (PubMed:11418101, PubMed:15979280). Related references: PMID:11418101 PMID:15979280
• The protein plays a key tumor suppressor role; loss of function is associated with multiple cancers (aliases: Mutated in multiple advanced cancers 1, Phosphatase and tensin homolog).
• PTEN localizes to the cytoplasm and nucleus, and is also found in PML bodies, dendritic spines, postsynaptic densities, and can be secreted.
• Expressed ubiquitously in adult tissues including heart, brain, placenta, lung, liver, muscle, kidney, and pancreas.
• Post-translational modifications such as phosphorylation, ubiquitination, and acetylation regulate its localization, stability, and tumor-suppressive activity.
• In cell migration, PTEN dephosphorylates FAK and cooperates with MAGI2 and DLC1 to modulate RHOA and RAC1, promoting cell polarization and directed movement (PubMed:11707428, PubMed:22279049, PubMed:26166433). Related references: PMID:11707428 PMID:22279049 PMID:26166433

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-200) of human PTEN, which includes the phosphatase domain; consider its potential for detecting full-length PTEN and isoforms that retain this region.
• For Western blotting, a signal at approximately 47 kDa is anticipated; validate expression in relevant cell or tissue lysates and include appropriate positive and negative controls.
• For immunohistochemistry and immunofluorescence, optimize antigen retrieval and blocking conditions; widespread tissue expression may require careful signal specificity verification.
• The antibody is reported to react with human, mouse, and rat; cross-reactivity should be confirmed in the intended experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw-material sourcing solutions for diagnostic manufacturers, laboratories, and research institutes. For studies on the PTEN/PI3K-AKT signaling pathway and tumor suppression, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing of rare-target raw materials. Partner with us to streamline your development from concept to clinic.