Anti-PTEN Rabbit Monoclonal Antibody for WB - P60484

Core Product Specifications and Parameters

Biological Background: PTEN Function and Localization

• PTEN (phosphatase and tensin homolog) is a dual-specificity protein phosphatase that dephosphorylates tyrosine-, serine-, and threonine-phosphorylated proteins. Related references: PMID:9187108 PMID:9256433 PMID:9616126
• It also acts as a lipid phosphatase, with a preference for phosphatidylinositol 3,4,5-trisphosphate (PIP3), removing the D3-phosphate to generate PIP2. Related references: PMID:16824732 PMID:26504226 PMID:9593664
• Central negative regulator of the PI3K-AKT/PKB signaling pathway; by dephosphorylating PIP3, it dampens AKT activation, thereby modulating cell cycle progression and survival. Related references: PMID:31492966 PMID:37279284
• Unphosphorylated PTEN cooperates with MAGI2 to suppress AKT1 activation; phosphorylation of PTEN switches its binding preference from p85 to DLC1, enabling RHOA-driven regulation of cell migration and polarity. Related references: PMID:11707428 PMID:26166433
• Dephosphorylates focal adhesion kinase (FAK) and inhibits cell migration, integrin-mediated cell spreading, and focal adhesion formation. Related references: PMID:22279049
• Involved in neurogenesis and synaptic plasticity; recruited to the postsynaptic density upon NMDA receptor activation to modulate long-term depression (LTD), and controls newborn neuron integration via AKT-mTOR signaling.
• Predominantly localizes to the cytoplasm and nucleus, with additional presence in PML bodies, dendritic spines, and the postsynaptic density; it can also be secreted. Expresses ubiquitously in adult tissues, including heart, brain, placenta, lung, liver, muscle, kidney, and pancreas.
• Recognized as a tumor suppressor; the nuclear monoubiquitinated form exhibits greater apoptotic potential than the cytoplasmic nonubiquitinated form. Related references: PMID:10468583 PMID:18716620

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (residues 304–403) of human PTEN; conservation across mouse and rat makes it likely suitable for multi-species detection in Western blot and IHC applications.
• In Western blot (WB), PTEN may produce multiple bands (~20 kDa, 47 kDa, and 65 kDa) due to isoforms and post-translational modifications; consider including phosphatase inhibitors in lysis buffer to preserve phosphorylation states.
• For immunohistochemistry (IHC-P), antigen retrieval conditions (e.g., citrate buffer pH 6.0 or EDTA pH 8.0) should be optimized empirically, and cross-reactivity with rodent tissues validated on relevant controls.
• In immunoprecipitation (IP), the rabbit IgG origin allows compatibility with standard Protein A/G beads; confirm the IgG subclass for optimal bead selection.
• For immunofluorescence/immunocytochemistry (IF/ICC), permeabilization with 0.1–0.5% Triton X-100 and blocking with 5% BSA may help reduce non-specific background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic developers and research laboratories with a comprehensive supply of IVD raw materials, specializing in critical oncology and neuroscience targets such as PTEN. From validated monoclonal antibodies and recombinant proteins to matched buffer systems and rare-target sourcing, we provide a streamlined procurement bridge to accelerate biomarker discovery and therapeutic assay development.