Anti-PTEN Polyclonal Antibody for IHC-P, IF/ICC, ELISA - P60484

Core Product Specifications and Parameters

Biological Background: PTEN Function and Localization

• PTEN (phosphatase and tensin homolog) is a dual-specificity phosphatase that dephosphorylates tyrosine-, serine-, and threonine-phosphorylated protein substrates. Related references: PMID:9187108 PMID:9256433 PMID:9616126
• It also functions as a lipid phosphatase, preferentially hydrolyzing the D3-phosphate of phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3), thereby antagonizing the PI3K-AKT signaling pathway to control cell cycle progression and survival. Related references: PMID:16824732 PMID:26504226 PMID:9593664
• PTEN is a major tumor suppressor; its loss leads to constitutive PI3K-AKT activation, promoting uncontrolled proliferation and contributing to many cancers. Related references: PMID:31492966 PMID:37279284
• Phosphorylation of PTEN modulates its binding to DLC1 and MAGI2, regulating cell migration and polarization; the unphosphorylated form cooperates with MAGI2 to suppress AKT1 activation. Related references: PMID:11707428 PMID:26166433
• In motile cells, PTEN dephosphorylates focal adhesion kinase (FAK) and suppresses lateral pseudopod formation, inhibiting cell migration and integrin-mediated cell spreading. Related references: PMID:22279049
• PTEN is found in the cytoplasm, nucleus, PML bodies, and secreted spaces; at postsynaptic densities and dendritic spines, it regulates synaptic plasticity and NMDA receptor-dependent long-term depression (LTD).
• PTEN is broadly expressed across adult tissues, including heart, brain, placenta, lung, liver, muscle, kidney, and pancreas, reflecting its fundamental regulatory roles.
• Post-translational modifications such as acetylation, ubiquitination, and phosphorylation control PTEN activity, stability, and subcellular localization; nuclear monoubiquitinated PTEN exhibits enhanced apoptotic potential. Related references: PMID:10468583 PMID:18716620

Experimental Guidance and Technical Tips

• The immunogen (aa 190–304) lies within the C2 and tail domains of PTEN, a region conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For IHC-P, optimize antigen retrieval (e.g., heat-induced epitope retrieval at pH 6 or 9) and include positive (e.g., prostate or breast tissue) and negative (PTEN-deficient tumor) controls.
• In IF/ICC, validate staining in known PTEN-expressing cell lines (e.g., HeLa, MCF7) and expect nuclear/cytoplasmic localization; co‑stain with organelle markers as needed.
• For ELISA, consider using the antibody for antigen detection and titrate carefully; pairing with a suitable capture antibody may enable sandwich formats.
• As a polyclonal antibody, verify lot-to-lot consistency in critical applications and use serum blocking to reduce background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to validated IVD raw materials, including polyclonal and monoclonal antibodies, optimized antibody pairs, and bulk ancillary reagents. This anti-PTEN polyclonal antibody supports research into tumor suppression, PI3K-AKT signaling, and neurobiology. Whether you need rare-target raw-material sourcing or technical support from concept to clinic, CamelBio streamlines your supply chain.