Anti-PTEN Monoclonal Antibody for WB, IHC-P, ELISA - P60484

Core Product Specifications and Parameters

Biological Background: PTEN Function and Localization

• PTEN is a dual-specificity protein phosphatase, dephosphorylating tyrosine-, serine- and threonine-phosphorylated proteins. Related references: PMID:9187108, PMID:9256433, PMID:9616126
• Also functions as a lipid phosphatase, removing the D3 phosphate of phosphatidylinositol phosphates, with preference for PtdIns(3,4,5)P3. Related references: PMID:16824732, PMID:26504226, PMID:9593664
• Antagonizes the PI3K-AKT/PKB signaling pathway, modulating cell cycle progression and cell survival. Related references: PMID:31492966, PMID:37279284
• Plays a role in cell migration: suppresses lateral pseudopods, dephosphorylates focal adhesion kinase, and regulates RHOA/RAC1 activation. Related references: PMID:22279049, PMID:26166433
• Subcellular localization: found in cytoplasm, nucleus, PML bodies, dendritic spines, postsynaptic density, and can be secreted.
• Expressed at relatively high levels in all adult tissues, including heart, brain, placenta, lung, liver, muscle, kidney, and pancreas.
• Post-translational modifications and keywords: acetylation, phosphorylation, ubiquitination; acts as tumor suppressor, involved in apoptosis, neurogenesis.
• An alternative isoform functions as a kinase, promoting mitochondrial COX activity and ATP production.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 304–403) of human PTEN, covering the C2 domain critical for membrane binding; this design may enhance detection of full-length PTEN.
• For Western blot, expect a band around 47 kDa; consider using appropriate loading controls and validate in relevant cell lines (e.g., human, mouse, rat).
• IHC-P applications may require optimization of antigen retrieval; test staining in tissues known to express PTEN.
• ELISA application: the monoclonal nature may ensure high specificity, but validate in the sample matrix.
• The antibody is rabbit monoclonal, which may offer high affinity and low background; confirm optimal working dilutions by titration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and laboratories with streamlined access to high-quality IVD raw materials, including this anti-PTEN monoclonal antibody for cancer and cell signaling research. Our comprehensive supply chain supports validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. Discover a seamless path from concept to clinic with CamelBio's integrated services and technical support.