Anti-PTEN Monoclonal Antibody for WB, IF/ICC, IP, ELISA - P60484

Core Product Specifications and Parameters

Biological Background: PTEN Function and Localization

• PTEN (phosphatase and tensin homolog) is a dual-specificity protein phosphatase and lipid phosphatase, acting as a critical tumor suppressor. The immunogen corresponds to amino acids 1-403 of the canonical isoform (UniProt P60484).
• As a lipid phosphatase, PTEN dephosphorylates phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) to antagonize the PI3K-AKT/PKB signaling pathway, thereby modulating cell cycle progression, survival, and metabolism. Related references: PMID:31492966 PMID:37279284
• It also functions as a protein phosphatase, dephosphorylating tyrosine-, serine-, and threonine-phosphorylated proteins, impacting diverse signaling cascades. Related references: PMID:9187108 PMID:9256433 PMID:9616126
• PTEN localizes to multiple subcellular compartments, including the cytoplasm, nucleus, PML bodies, dendritic spines, postsynaptic density, and can be secreted, reflecting its multifaceted roles.
• It is expressed at relatively high levels in all adult tissues, including heart, brain, placenta, lung, liver, muscle, kidney, and pancreas.
• PTEN plays a key role in cell migration by dephosphorylating focal adhesion kinase and interacting with DLC1/TNS3 complexes to regulate RHOA and RAC1 activation at the leading edge and posterior of migrating cells. Related references: PMID:22279049 PMID:26166433
• Post-translational modifications such as monoubiquitination influence PTEN's subcellular localization and apoptotic function; nuclear monoubiquitinated PTEN exhibits greater apoptotic potential. Related references: PMID:10468583 PMID:18716620

Experimental Guidance and Technical Tips

• The immunogen encompasses nearly full-length human PTEN (aa 1-403), so the antibody is expected to recognize total PTEN regardless of phosphorylation state. For Western blot, consider including phosphatase inhibitors in your lysis buffer to preserve endogenous PTEN phosphorylation patterns.
• PTEN migrates at approximately 47 kDa on SDS-PAGE; use appropriate molecular weight markers and run proper positive/negative controls (e.g., PTEN-knockout cell lysates) to confirm band identity.
• For immunoprecipitation, test different antibody-to-lysate ratios and incubation conditions to optimize capture efficiency. The antibody's performance in IP has been demonstrated; however, individual experimental systems may require optimization.
• In immunofluorescence/ICC, PTEN exhibits both cytoplasmic and nuclear staining. Fixation and permeabilization methods can significantly affect localization patterns; validate the staining in your cell line with known PTEN expression profiles and use isotype controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, offering validated monoclonal and polyclonal antibodies, bulk ancillary reagents, and technical support for diagnostic and life-science research. This anti-PTEN rabbit monoclonal antibody exemplifies our portfolio for oncology and cellular signaling targets. For laboratories and manufacturers seeking consistent, high-quality research tools or custom-developed raw materials, CamelBio streamlines procurement from concept to clinic.