Anti-PTCH1 Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - Q13635

Core Product Specifications and Parameters

Biological Background: PTCH1 Function and Localization

• Protein patched homolog 1 (PTCH1) acts as a receptor for hedgehog morphogens (SHH, IHH, DHH) and negatively regulates smoothened (SMO) signaling by preventing SMO access to cholesterol. Related references: PMID:21537345, PMID:29954986, PMID:29995851

• Functions as a cholesterol transporter, depleting cholesterol from the outer leaflet of the plasma membrane to inhibit SMO activation. Hedgehog binding inhibits this transporter activity, thereby promoting SMO signaling. Related references: PMID:31555730, PMID:37611095, PMID:31548691

• Possesses tumor suppressor activity; its inactivation is considered a necessary step for tumorigenesis. Related references: PMID:8658145

• Widely expressed in adult tissues including brain, lung, liver, heart, placenta, skeletal muscle, pancreas, and kidney; detected in tumor cells but not in normal skin.

• Localizes to the cell membrane and primary cilium membrane, consistent with its role in cilium-dependent hedgehog signaling.

• Undergoes post-translational modifications such as glycosylation, phosphorylation, and ubiquitination. Alternative splicing generates multiple isoforms, and mutations are associated with holoprosencephaly and basal cell nevus syndrome.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fragment spanning the C-terminal region (aa 1214–1312), which may recognize full-length PTCH1 (~161 kDa) in Western blotting.
• For immunohistochemistry (IHC) and immunofluorescence (IF), consider verifying specificity using positive control tissues such as brain, lung, or tumor samples where PTCH1 is known to be expressed.
• Cross-reactivity with mouse and rat allows application in rodent model studies; however, validation in the desired species and experimental system is recommended.
• As a polyclonal antibody, lot-to-lot consistency should be confirmed for quantitative assays.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, technical services, and consulting—supporting projects from concept to clinic. This anti-PTCH1 polyclonal antibody supports research into hedgehog signaling and cancer biology. Beyond single antibodies, we offer validated antibody pairs, optimized monoclonal/polyclonal reagents, bulk ancillary reagents, and expert sourcing for rare target raw materials to accelerate your assay development and manufacturing.