Anti-PTCH1 Polyclonal Antibody for WB, IF-P, ELISA - Q13635

Core Product Specifications and Parameters

Biological Background: PTCH1 Function and Localization

• PTCH1 is a receptor for hedgehog morphogens (SHH, IHH, DHH) and a negative regulator of the smoothened signaling pathway. Related references: PMID:21537345 PMID:29954986 PMID:29995851
• In the absence of hedgehog, PTCH1 inhibits SMO by depleting cholesterol from the outer leaflet of the plasma membrane, preventing cholesterol from binding to SMO's CRD region. Related references: PMID:31555730 PMID:37611095
• Hedgehog binding inhibits PTCH1's cholesterol transporter activity, enabling SMO activation and downstream signal transduction. Related references: PMID:31548691
• PTCH1 acts as a tumor suppressor; its inactivation is a crucial step in tumorigenesis. Related references: PMID:8658145
• It localizes to the cell membrane and the primary cilium membrane, consistent with its role in hedgehog signaling.
• Expression is observed in adult brain, lung, liver, heart, placenta, skeletal muscle, pancreas, and kidney, and in tumor cells but not normal skin.
• PTCH1 undergoes alternative splicing and post-translational modifications, including glycosylation, phosphorylation, and ubiquitination.
• Mutations in PTCH1 are associated with holoprosencephaly and other developmental disorders.

Experimental Guidance and Technical Tips

• This antibody was raised against a recombinant fragment corresponding to amino acids 770-970 of human PTCH1. Consider the immunogen region when evaluating epitope accessibility.
• For Western blotting, the predicted band size is ~161 kDa. Validate the antibody in a relevant cell or tissue lysate and use appropriate molecular weight markers.
• In immunofluorescence, PTCH1 localizes to the cell membrane and primary cilium. Ensure proper fixation and permeabilization protocols for membrane and cilium proteins.
• Cross-reactivity with mouse and rat allows the antibody to be used in those species; however, validate specificity in the target species.
• For ELISA, optimize coating conditions and antibody concentrations using the recombinant protein as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, supporting researchers and diagnostic manufacturers from early discovery to clinical application. This anti-PTCH1 antibody is ideal for investigations into hedgehog signaling, tumor biology, and developmental disorders. Through our global supply network, we can provide validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and assistance in sourcing rare-target raw materials to streamline your assay development.