Anti-PTCD3 Polyclonal Antibody for WB, IF/ICC, ELISA - Q96EY7

Core Product Specifications and Parameters

Biological Background: PTCD3 Function and Localization

• PTCD3 encodes the mitochondrial small ribosomal subunit protein mS39 (also known as 28S ribosomal protein S39, MRP-S39), a pentatricopeptide repeat (PPR) domain-containing protein critical for mitochondrial translation.
• It functions as a mitochondrial RNA-binding protein, contributing to the assembly, stability, or function of the mitochondrial ribosome and regulating mitochondrial protein synthesis.
• PTCD3 is a component of the small 28S subunit of the mitoribosome and belongs to the mitochondrial ribosomal protein family.
• The protein localizes exclusively to the mitochondrion, consistent with its role in organellar translation and energy metabolism.
• Post-translational modifications include acetylation, and alternative splicing generates multiple isoforms.
• PTCD3 is abundantly expressed in tissues with high energy demands, such as testes, skeletal muscle, and heart, underscoring its importance in mitochondrial function.
• Mutations in PTCD3 are linked to primary mitochondrial disease (e.g., COXPD51), highlighting its essential role in oxidative phosphorylation.
• UniProt keywords include: 3D-structure, Acetylation, Alternative splicing, Mitochondrion, Primary mitochondrial disease, Ribonucleoprotein, Ribosomal protein, RNA-binding, rRNA-binding, Transit peptide, Translation regulation.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 450–689 of human PTCD3, a region that may present conformational epitopes; consider using both reducing and non-reducing conditions in WB to assess specificity.
• As a mitochondrial protein, efficient extraction requires detergents for membrane solubilization; mitochondrial enrichment can improve detection sensitivity.
• The predicted molecular weight is ~79 kDa; validate band size in positive controls with high mitochondrial content (e.g., heart, skeletal muscle, testes) from human, mouse, or rat.
• For IF/ICC, include a permeabilization step to access mitochondria; co-staining with a mitochondrial marker is recommended.
• ELISA applications may need optimization of coating antigen and antibody dilution; recombinant protein or peptide fragments can serve as standards.
• Cross-reactivity is indicated for mouse and rat, but verify sequence homology in the immunogen region for other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports IVD manufacturers and research labs with a comprehensive portfolio of mitochondrial research reagents, including validated antibodies, protein standards, and ancillary materials. This anti-PTCD3 polyclonal antibody exemplifies our commitment to providing high-quality raw materials for mitochondrial disease diagnostics and translational studies. From single antibodies to bulk custom orders, CamelBio streamlines your sourcing for biomarker development and assay optimization, backed by technical consultation and supply chain expertise.