Anti-PTBP1 Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P26599

Core Product Specifications and Parameters

Biological Background: PTBP1 Function and Localization

• PTBP1, also known as heterogeneous nuclear ribonucleoprotein I (hnRNP I), is a 57–60 kDa RNA-binding protein that plays essential roles in pre-mRNA splicing and the regulation of alternative splicing events.
• It binds to polypyrimidine tracts of introns and may promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA, facilitating U2 snRNP recruitment to the pre-mRNA.
• PTBP1 represses the splicing of MAPT/Tau exon 10 (PubMed:15009664).
  Related references: PMID:15009664
• It regulates CFTR exon 9 skipping by binding to the polypyrimidine-rich controlling element (PCE), thereby antagonizing TIA1-mediated exon inclusion (PubMed:14966131).
  Related references: PMID:14966131
• PTBP1 controls pyruvate kinase PKM alternative splicing by repressively binding to a polypyrimidine tract flanking exon 9, inhibiting exon 9 inclusion and promoting the production of the PKM M2 isoform (PubMed:20010808).
  Related references: PMID:20010808
• Upon infection by picornaviruses, PTBP1 binds to the viral internal ribosome entry site (IRES) and stimulates IRES-mediated translation (PubMed:21518806).
  Related references: PMID:21518806
• PTBP1 localizes predominantly to the nucleus, consistent with its functions in nuclear pre-mRNA processing.
• The protein undergoes various post-translational modifications, including acetylation and phosphorylation, and is subject to ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to the N-terminal region (amino acids 1–100) of human PTBP1 (NP_114368.1). This region is expected to be present in the full-length protein.
• For western blot (WB) analysis, a specific band at approximately 60 kDa is anticipated. Validate the antibody with appropriate positive and negative controls in your sample system.
• In immunofluorescence (IF/ICC) and immunohistochemistry (IHC-P), PTBP1 is expected to localize to the nucleus. Use DAPI or a nuclear marker co-stain to confirm nuclear signal.
• Performance may vary depending on sample preparation, fixation, and antigen retrieval conditions. Optimization is recommended for each application.
• This antibody cross-reacts with human, mouse, and rat PTBP1. Verify cross-reactivity in your specific model organism if used beyond these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing partner for IVD raw materials, supporting diagnostic manufacturers, laboratories, and research institutes in developing and scaling assays. For researchers studying RNA splicing, alternative splicing regulation, and RNA-binding proteins, CamelBio offers validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing. Our portfolio, including high-quality targets like PTBP1, is designed to accelerate your project from concept to clinic.