Anti-PSRC1 Rabbit Polyclonal Antibody for WB, ELISA - Q6PGN9

Core Product Specifications and Parameters

Biological Background: PSRC1 Function and Localization

• PSRC1 (Proline/serine-rich coiled-coil protein 1), also known as DDA3 or FP3214, is a mitotic spindle-associated protein.
• It is required for normal progression through mitosis, facilitating proper chromosome congression at the metaphase plate and ensuring normal rates of chromosomal segregation during anaphase.
• PSRC1 regulates mitotic spindle dynamics by increasing the turnover of microtubules on metaphase spindles, contributing to the generation of normal tension across sister kinetochores.
• It recruits the microtubule depolymerase KIF2A and the spindle pole protein ANKRD53 to the mitotic spindle and spindle poles, thereby modulating spindle assembly and stability.
• Subcellularly, PSRC1 localizes to the cytoplasm, the mitotic spindle, and spindle poles during cell division.
• Widely expressed in adult and fetal tissues, with highest levels in the adult brain and fetal thymus; it is not detected in adult skeletal muscle.
• PSRC1 may also participate in p53/TP53-regulated growth suppression, linking cell cycle control to tumor suppression pathways.

Experimental Guidance and Technical Tips

• For Western blot, consider testing a range of dilutions on whole cell lysates from HeLa or HEK293T cells, which express PSRC1. The expected band size is approximately 39 kDa.
• For ELISA applications, the antibody can serve as a capture or detection reagent; however, pairing with a monoclonal antibody may improve specificity in sandwich formats.
• Due to the polyclonal nature, lot-to-lot consistency should be verified if used in quantitative assays.
• Since cross-reactivity is confirmed only for human, validate the antibody in the relevant sample system before applying to other species.

CamelBio: Your One-Stop Sourcing Bridge

For researchers and diagnostic developers working on cell cycle regulation, mitotic spindle biology, or cancer biomarkers, CamelBio provides reliable access to high-quality antibodies like this anti-PSRC1 polyclonal antibody. Our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to support your assay development from concept to clinic. Trust CamelBio as your strategic partner for one-stop procurement of critical IVD raw materials.