Anti-PSRC1 Polyclonal Antibody for WB, ELISA - Q6PGN9

Core Product Specifications and Parameters

Biological Background: PSRC1 Function and Localization

• PSRC1 (Proline/serine-rich coiled-coil protein 1), also known as DDA3, is a mitotic regulator required for normal chromosome congression and segregation during mitosis.
• It plays a role in regulating mitotic spindle dynamics by increasing microtubule turnover at metaphase spindles, contributing to proper tension across sister kinetochores.
• PSRC1 recruits KIF2A and ANKRD53 to the mitotic spindle and spindle poles, facilitating correct spindle function.
• It may participate in p53/TP53-regulated growth suppression, linking mitotic regulation to tumor suppression pathways.
• Subcellularly, PSRC1 localizes to the cytoplasm, specifically associating with the mitotic spindle and spindle poles.
• The protein is widely expressed in adult and fetal tissues, with highest levels in the adult brain and fetal thymus; it is absent in adult skeletal muscle.
• PSRC1 is a phosphoprotein with a coiled-coil domain and repeat regions, and is subject to alternative splicing.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (aa 1-215) of human PSRC1, covering a major portion of the protein. Confirm reactivity with full-length PSRC1 in your experimental system, especially for mouse samples where homology may influence binding.
• For Western blotting, the expected molecular weight is ~39 kDa (as indicated in product specifications). Use mitotically enriched cell lysates or tissues with high PSRC1 expression (e.g., brain) to facilitate detection.
• In ELISA, the recombinant immunogen can serve as a positive control. As a polyclonal antibody, consider potential lot-to-lot variation; validation in the intended assay format is recommended.
• Cross-reactivity with human and mouse is reported; when working with other species, verify sequence homology.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material sourcing, accelerating your path from research to regulated diagnostics. This anti-PSRC1 polyclonal antibody, targeting a key mitotic regulator, complements our broad portfolio of cell cycle and oncology markers. In addition to this product, we supply validated antibody pairs, optimized monoclonal reagents, and ancillary bulk materials, with tailored support for custom or rare-target requests to streamline your assay development.