Anti-PSPH Rabbit Polyclonal Antibody for WB and ELISA - P78330

Core Product Specifications and Parameters

Biological Background: PSPH Function and Localization

• PSPH (phosphoserine phosphatase) catalyzes the dephosphorylation of O-phospho-L-serine to L-serine, the last irreversible step in L-serine biosynthesis from carbohydrates. Related references: PMID:12213811 PMID:14673469 PMID:15291819
• The product L-serine is utilized in protein synthesis, amino acid production, nucleotide metabolism, glutathione synthesis, and can be racemized to D-serine, a neuromodulator. Related references: PMID:14673469
• PSPH may also act on O-phospho-D-serine (Probable).
• Subcellular localization is cytoplasmic, predominantly in the cytosol.
• The protein undergoes acetylation, and genetic variants in PSPH are associated with disease.

Experimental Guidance and Technical Tips

• The immunogen corresponds to full-length human PSPH (amino acids 1–225), which may provide recognition of the endogenous protein across tested species (human, mouse, rat).
• For Western blot (WB), consider using whole-cell lysates from relevant tissues or cell lines, verifying the expected band around 25 kDa.
• In ELISA, the antibody can be used for detection of recombinant or native PSPH; optimize coating and detection conditions.
• Always validate antibody performance in your specific experimental system, including appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, from concept to clinic. For researchers investigating serine metabolism or developing diagnostic assays targeting PSPH, we provide validated antibody pairs, bulk ancillary reagents, and custom sourcing of rare targets. Our comprehensive supply chain ensures timely delivery of high-quality reagents to accelerate your projects.