Anti-PSPC1 Polyclonal Antibody for WB, IHC-P, IP, ELISA - Q8WXF1

Core Product Specifications and Parameters

Biological Background: PSPC1 Function and Localization

• PSPC1 (Paraspeckle component 1) is an RNA-binding protein essential for the assembly and integrity of nuclear paraspeckles, subnuclear structures involved in gene regulation. Related references: PMID:22416126
• It binds to poly(A), poly(G), and poly(U) RNA homopolymers, indicating a preference for polyadenylated and purine-rich RNA sequences. Related references: PMID:22416126
• PSPC1 cooperates with NONO and SFPQ to modulate androgen receptor-mediated gene transcription, highlighting its role in hormone-dependent signaling.
• It acts as a circadian clock regulator by repressing the CLOCK-BMAL1 heterodimer's transcriptional activity, linking RNA-binding protein function to biological rhythms.
• PSPC1 participates in the innate immune response to DNA viruses by forming the HDP-RNP complex, which facilitates IRF3 phosphorylation and activates the cGAS-STING pathway. Related references: PMID:28712728
• The protein localizes primarily to nuclear speckles, the nucleolus, and the nuclear matrix, with some cytoplasmic presence, suggesting dynamic shuttling or context-dependent functions.
• Post-translational modifications include acetylation, methylation, and phosphorylation, which may regulate its activity, interactions, and subcellular localization.
• PSPC1 is broadly expressed in tissues such as pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, and heart, indicating its widespread biological importance.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1-70 of human PSPC1, an N-terminal region that may be conserved across species. For Western blot, a major band at approximately 59 kDa is expected. Consider using a positive control lysate from human, mouse, or rat cell lines.
• This antibody is suitable for multiple applications, including WB, IHC-P, IP, and ELISA. Each application may require distinct optimization of antibody dilution and incubation conditions. Validate in the relevant sample system, and perform blocking and washing steps as appropriate.
• Due to the nuclear localization of PSPC1, consider including appropriate nuclear and cytoplasmic fractionation or imaging controls to confirm subcellular signal specificity in IHC-P and immunofluorescence assays.
• Cross-reactivity is expected with mouse and rat PSPC1, but sequence identity of the immunogenic region in these species should be verified if quantitative comparative studies are planned.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. For researchers studying paraspeckle biology, transcriptional regulation, or innate immunity, we offer reliable access to anti-PSPC1 antibodies and can assist in sourcing rare-target raw materials tailored to your diagnostic or laboratory needs. Contact us to streamline your procurement process from concept to clinic.