Anti-PSMG1 Polyclonal Antibody for WB, IHC-P, ELISA - O95456

Core Product Specifications and Parameters

Biological Background: PSMG1 Function and Localization

• PSMG1, also known as Proteasome assembly chaperone 1, is a 32854 Da protein encoded by the PSMG1 gene on chromosome 21, with alternative names including PAC1, DSCR2, and C21LRP.
• It functions as a chaperone that promotes the assembly of the 20S proteasome by forming a heterodimer with PSMG2; this dimer binds to PSMA5 and PSMA7 subunits, facilitating alpha-ring formation and preventing premature dimerization.
• PSMG1 is localized in the cytoplasm and endoplasmic reticulum, consistent with its role in proteasome biogenesis.
• Tissue expression is widespread in fetal tissues, while in adults it is detected in brain, colon, leukocytes, breast, and testis, and in various proliferating cell lines.
• Post-translational modifications include acetylation and phosphorylation, suggesting regulation of its chaperone activity.
• The protein is classified under keywords such as chaperone, alternative splicing, and proteomics identification, highlighting its dynamic roles in protein homeostasis.

Experimental Guidance and Technical Tips

• The immunogen is a full-length recombinant human PSMG1 protein (amino acids 1-288). Consider that the antibody may recognize endogenous PSMG1 across human, mouse, and rat samples.
• For Western blotting, a band around 33 kDa is expected; include appropriate positive controls (e.g., HEK293T, HeLa cell lysates) and optimize loading to confirm specificity.
• In immunohistochemistry (IHC-P), validate staining in tissues with known PSMG1 expression, such as brain or testis, and include isotype controls to assess background.
• For ELISA applications, titrate the antibody to establish optimal detection limits and pair with a compatible detection system.
• Since PSMG1 is involved in proteasome assembly, consider functional assays to investigate its chaperone activity in cellular models.

CamelBio: Your One-Stop Sourcing Bridge

As a one-stop sourcing bridge, CamelBio provides diagnostic manufacturers and research groups with streamlined access to high-quality IVD raw materials, including validated antibodies, antigen proteins, and ancillary reagents. This anti-PSMG1 antibody supports studies in proteasome biology and Down syndrome-related pathways, and we can supply matched pairs, bulk polyclonal/monoclonal antibodies, and rare target sourcing to accelerate your assay development. Contact our team for customized procurement solutions tailored to your project needs.