Anti-PSMF1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q92530

Core Product Specifications and Parameters

Biological Background: PSMF1 (Proteasome inhibitor PI31 subunit) Function and Localization

• PSMF1 encodes the proteasome inhibitor PI31 subunit, a key regulator of the ubiquitin-proteasome system.
• PI31 inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome core particle.
• It additionally blocks proteasome activation mediated by the regulatory complexes PA700 (19S) and PA28.
• Subcellularly, PI31 localizes to the cytoplasm and the endoplasmic reticulum.
• The protein undergoes post-translational modifications, including acetylation, methylation, and phosphorylation, as indicated by UniProt keywords.
• PI31 has been identified through proteomics studies and structural biology efforts, contributing to our understanding of proteasome regulation.
• Its role in controlling proteasomal activity makes it a potential target in diseases involving protein homeostasis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human PSMF1 protein (amino acids 1-271), so the polyclonal antibody likely recognizes both native and denatured conformations.
• The antibody has been tested in WB, IHC-P, IF/ICC, and ELISA; for IHC-P, consider heat-induced antigen retrieval due to the cytoplasmic and ER localization.
• Cross-reactivity with mouse and rat supports the use of rodent models, but validate the antibody in your specific sample system.
• As a polyclonal antibody, lot-to-lot consistency should be monitored; consider pilot experiments with a new lot.
• For IF/ICC, appropriate permeabilization and blocking steps are essential to access intracellular PI31.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive IVD raw material partner, providing diagnostic manufacturers and labs with validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This PSMF1 antibody targets the proteasome inhibitor PI31, supporting research in protein degradation, cell cycle control, and disease mechanisms. Whether you need routine sourcing or rare target raw materials, CamelBio bridges the gap from concept to clinic with commercial flexibility and scientific support.