Anti-PSME3 Rabbit Polyclonal Antibody [KO Validated] for WB, IHC-P, IF/ICC, ELISA - P61289

Core Product Specifications and Parameters

Biological Background: PSME3 Function and Localization

• Proteasome activator complex subunit 3 (PSME3), also known as PA28gamma, REG-gamma, or Ki nuclear autoantigen, is a member of the 11S REG family of proteasome regulators.
• PSME3 forms a doughnut-shaped homoheptamer that associates with the 20S proteasome; it specifically activates the trypsin-like catalytic subunit while inhibiting the chymotrypsin-like and postglutamyl-preferring (PGPH) activities.
• PSME3 facilitates the MDM2-p53/TP53 interaction, promoting ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, thereby limiting p53 accumulation and inhibiting apoptosis after DNA damage.
• PSME3 mediates CCAR2 and CHEK2-dependent SIRT1 inhibition, linking it to cellular stress responses and metabolic regulation. Related references: PMID:25361978
• The protein localizes to both the nucleus and cytoplasm and is involved in cell cycle regulation.
• Post-translational modifications include acetylation and phosphorylation; PSME3 is also identified as a host-virus interaction factor.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (amino acids 200-254), which ensures generation of antibodies specific to PSME3. For applications requiring high specificity, consider antigen affinity purification.
• This polyclonal antibody has been validated for WB, IHC-P, IF/ICC, and ELISA. Standard protocols for each application are recommended; for WB, a dilution range of 1:500-1:2000 is a typical starting point, but optimal dilution should be determined empirically.
• When performing IHC-P or IF/ICC, include appropriate controls (e.g., peptide blocking) to confirm signal specificity, and validate in tissues/cell lines with known PSME3 expression.
• For ELISA, the antibody can be used as a capture or detection reagent; pair it with a compatible secondary antibody for detection.
• As PSME3 is expressed in human, mouse, and rat, this antibody is suitable for cross-species studies; however, always verify performance in the specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-PSME3 antibody supports proteasome, cell cycle, and apoptosis research, and we can assist in sourcing additional rare-target reagents to accelerate your assay development from concept to clinic.