Anti-PSME3 Polyclonal Antibody for WB, IHC-P - P61289

Core Product Specifications and Parameters

Biological Background: PSME3 Function and Localization

• PSME3, also known as PA28gamma, REG-gamma, or Ki nuclear autoantigen, is the gamma subunit of the 11S proteasome activator complex.
• It forms a doughnut-shaped homoheptamer that binds the 20S proteasome, enhancing trypsin-like activity while inhibiting chymotrypsin-like and postglutamyl-preferring activities.
• PSME3 plays a critical role in regulating apoptosis by facilitating MDM2-mediated ubiquitination and proteasomal degradation of p53/TP53, limiting p53 accumulation after DNA damage.
• It is also involved in cell cycle regulation and mediates CCAR2- and CHEK2-dependent inhibition of SIRT1. Related references: PMID:25361978
• Subcellularly, PSME3 localizes to both the nucleus and the cytoplasm.
• Post-translational modifications include acetylation and phosphorylation, and it is associated with host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody corresponds to a C-terminal peptide (aa 200–254) of human PSME3. Verify sequence homology if cross-reactivity with other species or isoforms is critical.
• For Western blot (WB), endogenous PSME3 migrates at approximately 30 kDa. Use appropriate loading controls and validate antibody specificity in your sample system.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimize antigen retrieval and permeabilization conditions. Include appropriate negative controls to confirm staining specificity.
• The antibody is also suitable for ELISA applications. Titrate the antibody to determine optimal working concentrations for your assay format.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic manufacturers and research labs with validated antibody pairs, monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw materials. For proteasome-related research, we supply a range of antibodies targeting regulatory subunits and catalytic components, ensuring reliable performance across multiple applications. Contact us to explore custom sourcing and technical support tailored to your workflow.