Anti-PSME2 Polyclonal Antibody (KO Validated) for WB and ELISA - Q9UL46

Core Product Specifications and Parameters

Biological Background: PSME2 Function and Localization

• PSME2, also known as PA28β, REG-beta, or 11S regulator complex subunit beta, is a subunit of the PA28 proteasome activator complex.
• It is implicated in immunoproteasome assembly and is required for efficient antigen processing, enhancing the generation of class I binding peptides by altering the cleavage pattern of the proteasome.
• The protein is a phosphoprotein and subject to acetylation, as indicated by proteomics identification.
• Structural information is available for PSME2, contributing to the understanding of its function.
• It is a reference proteome component, highlighting its fundamental role in cellular proteostasis.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human PSME2 protein (aa 1-239), so the antibody is expected to recognize the native protein with an apparent molecular weight of approximately 27 kDa in Western blot.
• For Western blot, consider using knockout-validated lysates as a negative control to confirm specificity.
• In ELISA applications, optimize coating conditions and antibody dilutions to achieve the desired signal-to-noise ratio.
• Since the antibody cross-reacts with mouse PSME2, it can be used in studies involving both human and mouse samples, but validation in the respective experimental system is recommended.
• As a polyclonal antibody, lot-to-lot consistency should be verified for critical experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target sourcing. We support diagnostic manufacturers and research laboratories in the immunoproteasome and antigen processing field with reliable, high-quality reagents and end-to-end technical services.