Anti-PSME1/PA28α Rabbit Monoclonal Antibody for WB, ELISA - Q06323

Core Product Specifications and Parameters

Biological Background: PSME1 Function and Localization

• PSME1 (Proteasome activator complex subunit 1) is a key component of the PA28 activator complex, also designated as PA28α, REG-alpha, or IFI5111.
• It plays a critical role in immunoproteasome assembly and is required for efficient antigen processing, particularly enhancing the generation of class I MHC-binding peptides by altering the cleavage pattern of the proteasome.
• The protein is also referred to by aliases including PA28A, REGalpha, and IGUP I-5111, and is encoded by the PSME1 gene (Gene ID: 5720).
• According to UniProt, PSME1 is annotated with keywords such as 3D-structure, alternative splicing, and proteasome, indicating its established structural characterization and splice variant forms.
• The theoretical molecular weight of the human PSME1 protein is approximately 28.7 kDa (UniProt), consistent with the observed ~29 kDa band in experimental setups.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–115), which may be exposed in native protein conformations; consider this design when planning blocking or neutralization experiments.
• For Western blot, standard protocols using whole‑cell lysates from human, mouse, or rat cells can be applied; validate band specificity via peptide competition or genetic knockdown.
• In ELISA applications, optimal antibody dilution and coating conditions should be determined empirically using recombinant protein or appropriate biological matrices.
• Cross‑reactivity with mouse and rat is reported; confirm reactivity in the relevant model system if using non‑human samples.

CamelBio: Your One‑Stop Sourcing Bridge

As a dedicated IVD raw‑material partner, CamelBio supports diagnostic manufacturers and research labs with validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This anti‑PSME1 monoclonal antibody exemplifies our offering in the immunoproteasome and antigen‑processing research area. For rare‑target sourcing or large‑scale production needs, our supply chain connects you from concept to clinic efficiently.