Anti-PSME1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q06323

Core Product Specifications and Parameters

Biological Background: PSME1 Function and Localization

• PSME1 encodes the proteasome activator complex subunit 1, also known as PA28alpha, REG-alpha, IGUP I-5111, and IFI5111, and is part of the 11S regulator complex of the proteasome.
• Alternative splicing generates multiple transcript variants, contributing to protein diversity.
• PA28alpha forms a heteroheptameric complex with PA28beta (PSME2) to enhance proteasomal cleavage, particularly promoting the generation of class I MHC-binding peptides.
• The activator complex is critical for immunoproteasome assembly and efficient antigen processing, linking proteasomal function to adaptive immunity.
• PSME1 is included in the reference proteome and has direct protein sequencing and 3D-structure data available, facilitating structural and functional studies.
• Its expression is upregulated by interferon gamma, highlighting a role in immune response modulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length human PSME1 (amino acids 1–249), which may support recognition of both human and mouse proteins given sequence similarity.
• For Western blot (WB), expect a dominant band near 29 kDa; additional isoforms may appear. Validate with appropriate positive controls.
• For immunofluorescence (IF/ICC), PSME1 localizes to proteasome complexes; optimize fixation and permeabilization protocols to preserve its native distribution.
• In ELISA applications, consider using recombinant PSME1 protein as a standard to assess antibody specificity and sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio specializes in supplying IVD raw materials and technical support for diagnostic development, from concept to clinic. This anti-PSME1 antibody serves research into proteasome biology and antigen presentation. We offer validated antibody pairs, bulk reagents, and custom sourcing for rare targets to streamline your assay development.