Anti-PSMD9 Polyclonal Antibody for WB, ELISA - O00233

Core Product Specifications and Parameters

Biological Background: PSMD9 Function and Localization

• PSMD9 (also known as p27 or Rpn4) is a non-ATPase regulatory subunit of the 19S regulatory particle of the 26S proteasome, a multi-catalytic proteinase complex.
• It functions as a chaperone specifically during the assembly of the 26S proteasome base subcomplex, part of the PA700/19S regulatory complex.
• During base assembly, PSMD9 forms an intermediate trimer complex with PSMC6 and PSMC3 (the modulator trimer complex) and is subsequently released as assembly progresses.
• The protein is expressed across all human tissues tested, with notably high expression in the liver and kidney.
• UniProt annotations classify PSMD9 as a phosphoprotein, and the gene undergoes alternative splicing, generating multiple isoforms that may contribute to regulatory diversity.

Experimental Guidance and Technical Tips

• For WB, expect a band near 25 kDa; optimize antibody dilution and loading controls for your sample type.
• Verify cross-reactivity in your model system—validated for human, mouse, and rat—by including appropriate positive and negative controls.
• Since the immunogen spans the N-terminal 223 amino acids, the antibody may recognize full-length and possibly spliced isoforms; confirm specificity via knockdown or peptide blocking if needed.
• For ELISA, consider testing a range of coating antigen concentrations and antibody dilutions to establish optimal conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and life science researchers to reliable IVD raw materials, with a focus on proteasome and protein homeostasis targets. This high-quality anti-PSMD9 polyclonal antibody complements our portfolio of validated antibody pairs, bulk ancillary reagents, and rare-target sourcing services. From discovery to clinical support, CamelBio streamlines your supply chain, helping you accelerate assay development and streamline procurement.