Anti-PSMD6 Polyclonal Antibody for WB, ELISA - Q15008

Core Product Specifications and Parameters

Biological Background: PSMD6 Function and Localization

• PSMD6, also known as Rpn7, S10, p44S10, or p42A, is a non-ATPase regulatory subunit of the 26S proteasome.
• As a component of the 19S regulatory particle, PSMD6 is essential for the ATP-dependent recognition and degradation of polyubiquitinated proteins.
• The 26S proteasome maintains protein homeostasis by eliminating misfolded, damaged, or short-lived regulatory proteins, thereby controlling diverse cellular processes.
• PSMD6 participates in key pathways such as cell cycle progression, apoptosis, and DNA damage repair through timely degradation of specific substrates.
• The protein is subject to alternative splicing, generating multiple isoforms that may modulate proteasome function.
• Structural studies have provided insights into the architecture of the 19S regulatory particle, including the Lid subcomplex where PSMD6 resides.
• Proteomic analyses have identified PSMD6 as a core proteasome component, validating its constitutive expression and purification in proteasome isolations.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein corresponding to amino acids 23-307 of human PSMD6. Since this region spans a large portion of the protein, the antibody may detect both native and denatured forms in ELISA and WB applications.
• For Western blotting, prepare whole-cell lysates from human, mouse, or rat cell lines. The predicted molecular weight of PSMD6 is approximately 46 kDa; a specific band at this size should be considered positive.
• In ELISA, the antibody can be used for antigen capture or detection. Optimize coating conditions and antibody dilutions using purified recombinant PSMD6 or proteasome-enriched fractions as standards.
• As PSMD6 is part of the 26S proteasome complex, co-immunoprecipitation experiments may be feasible to study protein-protein interactions, though this application is not listed and requires individual validation.
• For tissue or cell-specific expression studies, confirm PSMD6 expression levels via public databases (e.g., Human Protein Atlas) and validate in your sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined, one-stop sourcing solution for IVD raw materials. For proteasome research and drug target development, we provide validated antibodies, matched antigen pairs, and bulk ancillary reagents to support your assay development. Our expert team assists with rare target sourcing and technical consultation, bridging the gap from concept to clinic.