Anti-PSMD4 Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - P55036

Core Product Specifications and Parameters

Biological Background: PSMD4 Function and Localization

• PSMD4, also known as RPN10, S5A, AF, ASF, and antisecretory factor 1, is the 26S proteasome non-ATPase regulatory subunit 4, encoded by the PSMD4 gene (GeneID: 5710).
• It serves as a ubiquitin receptor subunit essential for ATP-dependent degradation of ubiquitinated proteins, recognizing and binding polyubiquitin chains to target substrates for proteasomal destruction.
• By facilitating the removal of misfolded, damaged, or unneeded proteins, PSMD4 plays a central role in protein homeostasis and participates in critical cellular processes including cell cycle progression, apoptosis, and DNA damage repair.
• PSMD4 exhibits a marked preference for longer polyubiquitin chains, enhancing the efficiency and selectivity of the ubiquitin-proteasome pathway.
• Post-translational modifications such as phosphorylation regulate PSMD4 function, and alternative splicing produces multiple isoforms with potentially distinct roles.

Experimental Guidance and Technical Tips

• For western blotting (WB), immunoprecipitation (IP), and ELISA, the immunogen covers full-length PSMD4 (amino acids 1-377); consider using positive controls from human or rat cell lines known to express PSMD4.
• In immunofluorescence (IF/ICC), appropriate fixation and permeabilization methods should be optimized, as PSMD4 may localize to both cytoplasmic and nuclear compartments depending on cell type and conditions.
• When designing co-IP experiments, note that Rpn10/PSMD4 interacts transiently with the proteasome lid; include appropriate elution buffers and confirm complex integrity with proteasome subunit antibodies.
• Dilution factors should be empirically determined; pilot experiments in the relevant detection system are recommended to establish optimal working concentrations for each application.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive inventory of IVD raw materials, including validated antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets like PSMD4. Whether you are developing assays for ubiquitin-proteasome pathway targets or scaling up proteasome-related IVD kits, CamelBio streamlines procurement with reliable supply chains and technical support—from concept to clinic.