Anti-PSMD4 Rabbit Monoclonal Antibody for WB, IF/ICC, ELISA - P55036

Core Product Specifications and Parameters

Biological Background: PSMD4 Function and Localization

• PSMD4 (26S proteasome non-ATPase regulatory subunit 4), also referred to as Rpn10, S5A, AF, ASF, MCB1, and pUB-R5, is a critical component of the 19S regulatory particle of the 26S proteasome.
• It functions as a primary ubiquitin receptor, using its ubiquitin-interacting motifs (UIMs) to capture and deliver polyubiquitinated substrates to the proteolytic core.
• Characterized by a preference for longer polyubiquitin chains (K48-linked tetraubiquitin or longer), PSMD4 promotes efficient and processive protein degradation.
• As part of the 26S proteasome, PSMD4 contributes to diverse biological processes, including cell cycle progression, apoptosis, gene expression, and DNA repair, through the regulated turnover of key proteins.
• The protein is subject to post-translational modifications; UniProt keywords note phosphorylation and isopeptide bond formation, which may modulate its activity or interaction with other proteasome subunits.
• Alternative splicing generates multiple isoforms, potentially expanding the range of substrates recognized or altering regulatory functions.
• The calculated molecular weight of PSMD4 is ~40.7 kDa, with an observed molecular weight of 41 kDa by SDS-PAGE.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide targeting amino acids 250–350; ensure that the epitope is accessible in your application setting (e.g., native vs. denatured conditions).
• For Western blot analysis, expect a band at 41 kDa in human, mouse, and rat cell or tissue lysates. Include appropriate positive and negative controls.
• In immunofluorescence/immunocytochemistry (IF/ICC), pre-test different fixation/permeabilization protocols to optimize detection of the predominantly cytoplasmic (and to a lesser extent nuclear) proteasome signal.
• For ELISA, consider using recombinant PSMD4 protein or synthetic peptide as a positive control, and validate the linearity of the detection under your assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, providing validated monoclonal antibodies like this anti-PSMD4 clone, along with matched antibody pairs, bulk ancillary reagents, and customized procurement solutions for the ubiquitin-proteasome research area. Our integrated supply chain supports diagnostic manufacturers and research labs in accelerating assay development from concept to clinical application, with reliable access to rare-target reagents and technical consultation.