Anti-PSMD3 Rabbit Polyclonal Antibody for WB, ELISA - O43242

Core Product Specifications and Parameters

Biological Background: PSMD3 Function and Localization

• PSMD3, also known as RPN3, S3, P58, or TSTA2, is the 26S proteasome non-ATPase regulatory subunit 3, a component of the regulatory particle of the 26S proteasome.
• It participates in the ATP-dependent degradation of ubiquitinated proteins, playing a key role in maintaining protein homeostasis by eliminating misfolded, damaged, or obsolete proteins.
• The 26S proteasome is involved in numerous cellular processes including cell cycle progression, apoptosis, and DNA damage repair, highlighting PSMD3’s broad regulatory importance.
• PSMD3 is subject to post-translational modifications, including phosphorylation and ubiquitination (Ubl conjugation), and the PSMD3 gene undergoes alternative splicing to generate multiple isoforms.
• The protein has a calculated molecular weight of approximately 61 kDa, consistent with the band observed in Western blotting.

Experimental Guidance and Technical Tips

• For Western blotting (WB), use whole cell or tissue lysates from human, mouse, or rat samples. Validate the antibody specificity with a knockdown or knockout control, especially given its polyclonal nature.
• A synthetic peptide immunogen ensures recognition of a specific epitope, but cross-reactivity with related family members cannot be excluded; verify with sequence alignment if necessary.
• For ELISA, immobilize the peptide immunogen or recombinant protein, and titrate the antibody for optimal detection. Consider direct or indirect detection strategies based on assay design.
• Since the antibody is polyclonal, it may exhibit lot-to-lot variability; always perform quality control with known positive and negative samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. For projects targeting the ubiquitin-proteasome pathway, CamelBio can support your development with reliable PSMD3 antibodies and related reagents, streamlining your path from concept to clinical application. Our expertise in rare target sourcing ensures you receive the right materials for robust assay development.