Anti-PSMD14 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O00487

Core Product Specifications and Parameters

Biological Background: PSMD14 Function and Localization

• PSMD14, also known as POH1 or RPN11, is a component of the 26S proteasome regulatory subunit, a large multiprotein complex responsible for ATP-dependent degradation of ubiquitinated proteins, crucial for protein homeostasis, cell cycle progression, apoptosis, and DNA repair. Related references: PMID:9374539 PMID:1317798

• It functions as a metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains within the 26S proteasome, a critical deubiquitinating activity required for substrate processing and recycling of ubiquitin. Related references: PMID:22909820

• In DNA damage repair, PSMD14 promotes non-homologous end joining (NHEJ) by removing Lys-63-linked polyubiquitin, facilitating JMJD2A/KDM4A chromatin retention and limiting TP53BP1 accumulation; it also enhances homologous recombination by supporting RAD51 loading. Related references: PMID:22909820

• Additionally, PSMD14 regulates macroautophagy by ensuring Golgi-to-ER retrograde transport via its deubiquitinating activity, preventing mislocalization of essential autophagy proteins and enabling proper autophagosome formation. Related references: PMID:32210007

• The protein is widely expressed, with highest levels in heart and skeletal muscle, reflecting its essential housekeeping role.

• Key structural and functional features include its metalloprotease domain with zinc binding, and its classification as a thiol protease; it is also a phosphoprotein and part of the ubiquitin conjugation pathway.

Experimental Guidance and Technical Tips

• The immunogen used corresponds to amino acids 160-300 of human PSMD14, a region predicted to be exposed and conserved across species, which may contribute to the antibody's cross-reactivity with mouse and rat.
• For Western blot (WB), the expected band is at ~35kDa; consider using appropriate loading controls and validate the signal with siRNA or knockout lysates to confirm specificity.
• In immunofluorescence/immunocytochemistry (IF/ICC), include negative controls (e.g., secondary antibody alone) and verify localization with organelle markers, as PSMD14 associates with the proteasome in both cytoplasmic and nuclear compartments.
• For ELISA, perform titration experiments to determine optimal coating antigen and antibody concentrations; the antibody's performance should be validated in the relevant sample matrix.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic developers and researchers with high-quality antibodies like this anti-PSMD14 rabbit polyclonal antibody, suited for biomarker discovery, proteasome research, and DNA repair pathway studies. We offer validated antibody pairs, optimized monoclonal/polyclonal reagents, and bulk ancillary materials to streamline your assay development from concept to clinical stages. For any rare or custom raw-material needs, CamelBio leverages its global network to ensure timely and reliable procurement, supporting your entire R&D workflow.