Anti-PSMD13 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q9UNM6

Core Product Specifications and Parameters

Biological Background: PSMD13 Function and Localization

• PSMD13, also known as Rpn9, S11, or p40.5, is a component of the 26S proteasome, a large multiprotein complex responsible for ATP-dependent degradation of ubiquitinated proteins.
• The 26S proteasome plays a critical role in maintaining protein homeostasis by eliminating misfolded or damaged proteins and regulating the turnover of proteins involved in cell cycle progression, apoptosis, and DNA damage repair.
• PSMD13 belongs to the proteasome regulatory particle non-ATPase subunit family and is a stoichiometric subunit of the 19S regulatory complex, which recognizes polyubiquitinated substrates and deubiquitinates them before translocation into the 20S core particle for degradation.
• The protein is subject to acetylation and alternative splicing, generating multiple isoforms that may have tissue-specific or functional differences.
• Subcellularly, PSMD13 localizes to both the cytoplasm and nucleus, consistent with the distribution of proteasomes throughout the cell.
• Genetic and biochemical studies have identified PSMD13 as a conserved proteasome subunit, with structural data available (3D-structure keyword) providing insights into its role in substrate processing.

Experimental Guidance and Technical Tips

• The immunogen was designed against the N-terminal region (aa 1-250) of human PSMD13; consider verifying that this region is accessible in native or fixed conformations.
• For Western blot (WB), a single band at approximately 43 kDa is expected; however, post-translational modifications or isoform variants may produce additional bands.
• In immunohistochemistry (IHC-P), test various antigen retrieval methods (e.g., citrate buffer, pH 6.0) and antibody dilutions to optimize signal-to-noise ratio.
• For immunofluorescence (IF/ICC), co-stain with a cytoplasmic or nuclear marker to confirm localization, and use appropriate fixation (e.g., 4% PFA) to preserve epitope.
• ELISA protocols can be developed using this polyclonal as either capture or detection antibody; pair with a validated monoclonal for sandwich ELISA.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing platform for IVD raw materials, offering validated monoclonal and polyclonal antibodies, antigen pairs, and bulk ancillary reagents. This anti-PSMD13 polyclonal antibody supports research into the ubiquitin-proteasome system and protein degradation pathways. For your diagnostic development needs, CamelBio can also assist in sourcing rare-target raw materials and provide technical consulting to accelerate your project from concept to clinic.