Anti-PSMC6 Rabbit Polyclonal Antibody for WB and ELISA - P62333

Core Product Specifications and Parameters

Biological Background: PSMC6 Function and Localization

• PSMC6, also known as 26S proteasome regulatory subunit 10B, RPT4 (AAA-ATPase subunit), and proteasome subunit p42, is encoded by the PSMC6 (SUG2) gene.
• It is a core component of the 19S regulatory particle of the 26S proteasome, where it belongs to the heterohexameric AAA-ATPase ring responsible for substrate unfolding and translocation.
• PSMC6 participates in the ATP-dependent degradation of polyubiquitinated proteins, playing a critical role in maintaining protein homeostasis by eliminating misfolded, damaged, or regulatory proteins.
• The proteasome, through PSMC6, is involved in numerous cellular processes including cell cycle progression, apoptosis, and DNA damage repair.
• PSMC6 localizes to both the cytoplasm and the nucleus, consistent with its role in regulated proteolysis across cellular compartments.
• Post-translational modifications of PSMC6 include acetylation and phosphorylation, which may modulate proteasome activity and assembly.
• The PSMC6 protein has a molecular weight of approximately 44 kDa (predicted 44173 Da from the primary sequence).

Experimental Guidance and Technical Tips

• The immunogen used spans amino acids 20–150 of human PSMC6, an N-terminal region; the antibody may detect both full-length and truncated forms.
• For Western blot, consider using lysates from cells treated with proteasome inhibitors versus untreated controls, and validate band size against known PSMC6 molecular weight.
• For ELISA, coating with the recombinant immunogen or purified 26S proteasome can be used; cross-reactivity with mouse PSMC6 should be verified in the relevant experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, and comprehensive ancillary reagents. For proteasome research, we supply high-quality antibodies like this anti-PSMC6 polyclonal antibody to support your diagnostic and life science projects. Contact our team for bulk orders and custom sourcing solutions tailored to your needs.