Anti-PSMC5 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P62195

Core Product Specifications and Parameters

Biological Background: PSMC5 Function and Localization

• PSMC5, also known as 26S proteasome regulatory subunit 8, is a component of the 19S regulatory particle of the 26S proteasome, a multiprotein complex responsible for ATP-dependent degradation of ubiquitinated proteins.
• The protein is a member of the AAA (ATPases associated with diverse cellular activities) family and forms part of the heterohexameric ring that unfolds target proteins, facilitating their translocation into the proteolytic core particle for degradation.
• PSMC5 is involved in fundamental cellular processes including protein homeostasis, cell cycle progression, apoptosis, and DNA damage repair through its role in regulated proteolysis.
• The protein localizes to both the cytoplasm and the nucleus, reflecting the distribution of proteasome complexes throughout the cell.
• Post-translational modifications of PSMC5 include acetylation and phosphorylation, which may regulate its activity or interactions.
• Alternative splicing of the PSMC5 gene results in multiple transcript variants, contributing to functional diversity.
• The protein contains an ATP-binding domain and nucleotide-binding sites, essential for its ATPase activity and function in substrate unfolding.
• PSMC5 has also been identified as thyroid hormone receptor-interacting protein 1 (TRIP1), suggesting potential roles beyond proteasomal degradation in transcriptional regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a central region (aa 127-406) of PSMC5, ensuring recognition of native protein conformations; this design may enhance specificity across human, mouse, and rat species.
• For Western blotting (WB), consider using whole cell lysates from human, mouse, or rat cell lines; the predicted molecular weight is 46 kDa, but verify band migration under your experimental conditions.
• In immunofluorescence/immunocytochemistry (IF/ICC), cells may require permeabilization to detect both cytoplasmic and nuclear pools of PSMC5; optimize fixation and permeabilization protocols for your cell type.
• For ELISA applications, the antibody can be used as a capture or detection reagent; validate with recombinant PSMC5 or cell lysates to determine optimal coating concentrations.
• Since PSMC5 is highly conserved, this antibody is expected to cross-react with mouse and rat samples; however, we recommend verifying reactivity in your specific sample system.
• Use appropriate protease inhibitors during sample preparation to maintain proteasome integrity and prevent target degradation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a comprehensive sourcing solution for IVD raw materials, covering every stage from concept to clinic. With this anti-PSMC5 polyclonal antibody, researchers gain reliable access to a well-characterized reagent for proteasome and ubiquitin-proteasome pathway investigations. Beyond this product, CamelBio can supply validated antibody pairs, optimized monoclonal or polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare-target raw materials, all backed by technical consultation to streamline your assay development and production workflows.