Anti-PSMC5 Rabbit Polyclonal Antibody for WB, ELISA - P62195

Core Product Specifications and Parameters

Biological Background: PSMC5 Function and Localization

• PSMC5 (Proteasome 26S subunit ATPase 5) is also known as RPT6, SUG1, TRIP1, and p45. It is an essential AAA-ATPase component of the 19S regulatory particle of the 26S proteasome.
• The 26S proteasome is an ATP-dependent protease complex responsible for degrading polyubiquitinated proteins, thereby maintaining cellular protein homeostasis.
• PSMC5, along with five other AAA-ATPases, forms a heterohexameric ring that unfolds and translocates substrate proteins into the 20S core particle for degradation.
• The proteasome, through PSMC5 activity, regulates numerous cellular processes including cell cycle progression, apoptosis, and DNA damage repair.
• PSMC5 localizes to both the cytoplasm and the nucleus, consistent with its role in protein degradation throughout the cell.
• Post-translational modifications of PSMC5 include acetylation and phosphorylation, which may modulate its activity and interactions.
• The PSMC5 gene undergoes alternative splicing, generating multiple transcript variants that may encode different isoforms.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 127–406 of human PSMC5. The resulting antibody may recognize multiple isoforms arising from alternative splicing. Verify in the relevant sample system.
• For Western blotting (WB), consider using reducing conditions and loading a positive control such as HeLa or HEK293 cell lysate. The expected molecular weight is approximately 46 kDa.
• When using mouse samples, confirm cross-reactivity due to the reported human–mouse reactivity; however, perform validation as sequence differences may affect affinity.
• For ELISA, optimize coating antigen and antibody concentrations. Recombinant PSMC5 protein or cell extracts can be used as targets.
• This polyclonal antibody can be applied in experiments investigating proteasome-mediated degradation, cell cycle, or apoptosis pathways. Pair with appropriate inhibitors (e.g., MG132) to modulate proteasome activity when needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For PSMC5-related research or assay development, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies like this anti-PSMC5 reagent, and bulk ancillary reagents. Whether you need rare-target raw-material sourcing or a reliable supplier for proteasome pathway components, CamelBio streamlines your procurement process to accelerate your projects.