Anti-PSMC5 Monoclonal Antibody for WB, IF/ICC, ELISA - P62195

Core Product Specifications and Parameters

Biological Background: PSMC5 Function and Localization

• PSMC5, also known as 26S proteasome regulatory subunit 8, SUG1, TRIP1, or p45, is an essential component of the 26S proteasome, a large ATP-dependent protease complex responsible for degrading ubiquitinated proteins.
• It is a member of the heterohexameric AAA-ATPase ring that sits atop the proteolytic core particle, where it unfolds target proteins and translocates them into the degradation chamber.
• Through its role in protein degradation, PSMC5 contributes to the maintenance of cellular protein homeostasis, removing misfolded, damaged, or regulatory proteins and thereby influencing cell cycle progression, apoptosis, and DNA damage repair.
• The protein localizes to both the cytoplasm and the nucleus, consistent with the broad distribution of the 26S proteasome.
• PSMC5 is subject to post-translational modifications, including phosphorylation and acetylation, which may regulate its assembly or activity within the proteasome complex.

Experimental Guidance and Technical Tips

• The immunogen used (a synthetic peptide corresponding to amino acids 1-100 of human PSMC5) suggests the antibody may recognize an N-terminal epitope, which could be conserved across species, consistent with the reported human, mouse, and rat cross-reactivity.
• For Western blotting (WB), the predicted molecular weight is 46 kDa; consider using appropriate protein markers and validate the banding pattern in your specific sample lysates.
• In immunofluorescence (IF/ICC) experiments, expect staining in both cytoplasmic and nuclear compartments; fixation and permeabilization conditions should be optimized to preserve the epitope.
• ELISA applications may benefit from coating conditions that maintain the conformational integrity of the target, as the antibody was raised against a linear peptide.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies IVD raw material procurement by delivering comprehensive solutions for all diagnostic development stages. This anti-PSMC5 monoclonal antibody supports research on proteasome biology and ubiquitin-dependent pathways, and we can supply validated antibody pairs, optimized bulk reagents, and rare-target sourcing to meet your project requirements. Partner with CamelBio to bridge your raw material needs with production-ready quality.