Anti-PSMC4 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P43686

Core Product Specifications and Parameters

Biological Background: PSMC4 Function and Localization

• PSMC4 encodes the 26S proteasome regulatory subunit 6B, also known as RPT3, TBP-7, and MIP224, and belongs to the AAA ATPase family.
• It is an essential component of the 19S regulatory particle of the 26S proteasome, where it forms part of a heterohexameric ring that unfolds and translocates ubiquitinated substrates into the 20S core for degradation.
• The 26S proteasome is central to maintaining cellular protein homeostasis, removing misfolded or damaged proteins and regulating proteins involved in cell cycle progression, apoptosis, and DNA damage repair.
• PSMC4 is localized in both the cytoplasm and the nucleus, consistent with its role in proteasomal degradation across cellular compartments.
• The protein undergoes post-translational modifications including acetylation and phosphorylation, which may modulate its activity or interactions.
• Alternative splicing of PSMC4 has been reported, potentially generating distinct isoforms with functional variations.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminus (aa 1–165) of human PSMC4; this region is conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blot, a band of approximately 47 kDa is expected. Use standard reducing and denaturing sample preparation conditions, and validate with appropriate positive and negative controls.
• In IF/ICC, optimize fixation and permeabilization protocols to ensure antibody access to both cytoplasmic and nuclear targets.
• For ELISA, the antibody can be used as a capture or detection reagent. Consider pairing with an appropriate detection antibody if developing a sandwich ELISA.
• As with any polyclonal antibody, lot-to-lot variability may occur; include internal reference standards for quantitative applications and verify performance in your specific assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we empower diagnostic manufacturers, labs, and research institutes with a streamlined sourcing platform for IVD raw materials. This anti-PSMC4 antibody supports research into the ubiquitin-proteasome system, protein homeostasis, and related diseases such as cancer and neurodegeneration. Beyond validated polyclonal antibodies, CamelBio offers an extensive portfolio of antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, accelerating your progression from concept to clinic.