Anti-PSMC4 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - P43686

Core Product Specifications and Parameters

Biological Background: PSMC4 Function and Localization

• PSMC4, also known as 26S proteasome regulatory subunit 6B, RPT3, TBP-7, or MIP224, is a core component of the 19S regulatory particle of the 26S proteasome, a large ATP-dependent protease complex.
• It belongs to the heterohexameric AAA-ATPase ring that unfolds and translocates ubiquitinated substrate proteins into the 20S core particle for degradation into peptides.
• The 26S proteasome is essential for maintaining protein homeostasis by degrading misfolded, damaged, or regulatory proteins, thereby playing a key role in cell cycle progression, apoptosis, and DNA damage repair.
• PSMC4 localizes to both the cytoplasm and nucleus, consistent with its role in degrading nuclear and cytoplasmic substrates.
• Post-translational modifications include acetylation and phosphorylation; alternative splicing generates multiple isoforms.
• Keywords from UniProt include ATP-binding, nucleus, cytoplasm, phosphoprotein, and proteasome, reflecting its energy-dependent function and subcellular distribution.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–165), providing a defined epitope for target recognition.
• For WB, expect a band at approximately 47 kDa; validate specificity using appropriate positive and negative controls.
• For IHC-P, consider antigen retrieval methods and test in known PSMC4-expressing tissues; cross-reactivity with mouse and rat enables orthologous studies.
• ELISA applications may require optimization of coating and detection conditions; verify linearity and sensitivity with recombinant protein standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement by offering validated monoclonal antibodies, antibody pairs, and bulk ancillary reagents for diagnostic development. For proteasome research, we provide anti-PSMC4 antibodies and tailored sourcing services to support your project from concept to commercialization. Our rare-target sourcing network can accelerate your biomarker or drug-discovery pipeline with reliable, research-grade materials.