Anti-PSMC1 Polyclonal Antibody for WB, IHC-P, ELISA - P62191

Core Product Specifications and Parameters

Biological Background: PSMC1 Function and Localization

• PSMC1, also known as 26S proteasome regulatory subunit 4 or RPT2, is a core subunit of the 26S proteasome complex critical for ATP-dependent degradation of ubiquitinated proteins.
• As a member of the heterohexameric AAA-ATPase ring, PSMC1 uses ATP hydrolysis to unfold protein substrates and translocate them into the proteolytic core for degradation.
• The 26S proteasome maintains protein homeostasis by eliminating misfolded, damaged, or regulatory proteins, thereby regulating cell cycle progression, apoptosis, and DNA damage repair.
• PSMC1 localizes to the cytoplasm, nucleus, and membrane, reflecting the proteasome's diverse cellular functions.
• Post-translational modifications of PSMC1 include acetylation, phosphorylation, and ubiquitination, which may modulate proteasome activity or substrate recognition.
• The protein is also associated with keywords such as Alternative splicing, Deafness, and Intellectual disability, hinting at its broader physiological significance.

Experimental Guidance and Technical Tips

• The immunogen spans a large region (aa 77–440), which may allow recognition of full-length PSMC1 and potentially cross-react with homologous proteins; validate specificity using knockout or siRNA knockdown controls in the appropriate cell lines.
• For Western blotting, a band at approximately 49 kDa is predicted; loading controls and molecular weight markers are recommended to confirm specificity.
• In immunohistochemistry (IHC-P), optimize antigen retrieval and blocking conditions for the tissue of interest, as PSMC1 localization may vary between nuclear and cytoplasmic compartments.
• ELISA assays may be used for quantitative detection; titration of both capture and detection antibodies is advised to achieve optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research institutions a streamlined sourcing solution for IVD raw materials, from validated antibodies like this anti-PSMC1 polyclonal antibody to complete reagent panels. Supporting proteasome and ubiquitin pathway research, we provide monoclonal and polyclonal antibodies, bulk ancillary reagents, and customized raw-material sourcing to accelerate your assay development and production.