Anti-PSMB9/LMP2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P28065

Core Product Specifications and Parameters

Biological Background: PSMB9/LMP2 Function and Localization

• Proteasome subunit beta type-9 (PSMB9), also known as low molecular mass protein 2 (LMP2), is a catalytic component of the immunoproteasome, a specialized proteasome involved in antigen processing.
• The proteasome is a multicatalytic proteinase complex that cleaves peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Related references: PMID:33727065 PMID:34819510
• Replacement of the constitutive subunit PSMB6 by PSMB9 in the immunoproteasome enhances the capacity to cleave model peptides after hydrophobic and basic residues, optimizing the generation of MHC class I-binding peptides.
• PSMB9 localizes to both the cytoplasm and the nucleus, reflecting its role in protein degradation and antigen presentation in multiple cellular compartments.
• Post-translational modifications include acetylation; alternative splicing generates multiple isoforms, and the protein is associated with disease variants such as PRAAS3 (proteasome-associated autoinflammatory syndrome 3).
• The protein plays a role in host-virus interactions and is classified as a threonine protease belonging to the T1B peptidase family.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–219 of human PSMB9/LMP2, covering a large N-terminal portion of the protein. This design may enable recognition of full-length and truncated forms; consider validating in your relevant sample system.
• For Western blot (WB), a band near 23 kDa is expected. Use appropriate positive controls (e.g., human immune cell lysates) and optimize blocking and antibody dilution empirically.
• For immunofluorescence (IF/ICC), confirm fixation and permeabilization conditions to maintain epitope accessibility; PSMB9 may exhibit both cytoplasmic and nuclear staining patterns.
• In ELISA applications, the polyclonal nature of the antibody may provide robust signal; pair with a matched detection antibody if developing a sandwich assay.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a streamlined channel for IVD raw-material procurement, from concept to clinic. Whether you need validated antibody pairs, optimized polyclonal antibodies like this anti-PSMB9/LMP2 reagent, bulk ancillary reagents, or rare target sourcing for immunoproteasome and antigen processing research, we provide reliable access to quality materials backed by technical and supply-chain support.