Anti-PSMB9/LMP2 Monoclonal Antibody for WB, IHC-P, ELISA - P28065

Core Product Specifications and Parameters

Biological Background: PSMB9/LMP2 Function and Localization

• The PSMB9 protein, also known as LMP2, is a catalytic subunit of the immunoproteasome, a multicatalytic proteinase complex that replaces the constitutive PSMB6 subunit.
• The proteasome cleaves peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH through an ATP-dependent mechanism (PubMed:33727065, PubMed:34819510). Related references: PMID:33727065 PMID:34819510
• This subunit is critically involved in antigen processing to generate class I MHC-binding peptides, and its incorporation enhances cleavage after hydrophobic and basic residues.
• Subcellular localization includes both cytoplasm and nucleus.
• Key post-translational modifications include acetylation; the protein is synthesized as a zymogen that undergoes autocatalytic activation.
• Alternative splicing generates multiple isoforms, and the gene is linked to disease variants such as PRAAS3 (proteasome-associated autoinflammatory syndrome 3).
• PSMB9 participates in host-virus interactions and is a component of the immunoproteasome, which is inducible by interferon-gamma.

Experimental Guidance and Technical Tips

• The immunogen used was a recombinant fusion protein corresponding to amino acids 125–219 of human PSMB9/LMP2 (P28065), which targets a region within the mature proteasome subunit. Users should consider this when designing detection or blocking strategies.
• In Western blot (WB), expect a specific band at approximately 23 kDa (the molecular weight of the mature active form). Loading controls and molecular weight markers are essential for accurate interpretation.
• For immunohistochemical paraffin-embedded (IHC-P) staining, antigen retrieval and blocking conditions may need optimization; PSMB9 expression can vary with immune activation status.
• In ELISA applications, the antibody may serve as a capture or detection reagent; titration and pairing with appropriate standards are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing bridge for IVD raw materials, offering this anti-PSMB9/LMP2 monoclonal antibody along with validated antibody pairs, bulk ancillary reagents, and technical support. For immunoproteasome research and diagnostic assay development targeting antigen processing pathways, CamelBio can also assist in sourcing rare-target raw materials to streamline your workflow from concept to clinic.