Anti-PSMB8 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P28062

Core Product Specifications and Parameters

Biological Background: PSMB8 Function and Localization

• PSMB8 (Proteasome subunit beta type-8), also known as LMP7, PSMB5i, and RING10, is a catalytic subunit of the immunoproteasome, a specialized form of the 26S proteasome.
• It replaces the constitutive subunit PSMB5 in the immunoproteasome, enhancing the capacity to cleave peptides after hydrophobic and basic residues, thereby promoting the generation of MHC class I-binding peptides for antigen presentation.
• PSMB8 plays a critical role in the generation of spliced antigenic peptides, produced by the ligation of two separate cleavage products, a process essential for expanding the diversity of presented antigens (PubMed:27049119). Related references: PMID:27049119
• The protein is involved in apoptosis by mediating the degradation of the anti-apoptotic protein MCL1, and it serves as a major component of interferon gamma-induced sensitivity.
• PSMB8 is also implicated in inflammatory response pathways and is required for the differentiation of preadipocytes into adipocytes, linking the immunoproteasome to metabolic regulation.
• Subcellularly, PSMB8 localizes to both the cytoplasm and the nucleus, consistent with proteasome distribution.
• Post-translational modifications include phosphorylation (as annotated in UniProt keywords), and the protein undergoes alternative splicing, producing isoforms with distinct functions (e.g., isoforms E1 and E2 in cancer cells).

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 163-272 of human PSMB8, corresponding to the C-terminal region. This design may enhance specificity for detecting endogenous PSMB8 across species.
• For Western blotting, a band at approximately 30 kDa is expected. Validate by using appropriate positive and negative controls in your sample system.
• In immunofluorescence/immunocytochemistry, anticipate cytoplasmic and nuclear staining. Optimize fixation and permeabilization conditions for best signal.
• For ELISA applications, consider pairing this polyclonal antibody with a monoclonal capture antibody for sandwich ELISA development; validate with recombinant PSMB8 or cell lysates.
• Since PSMB8 expression can be induced by interferon gamma, you may need to stimulate cells to achieve detectable levels in some cell types.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-PSMB8, and bulk ancillary reagents, serving diagnostic manufacturers and research laboratories. Our expertise in immunoproteasome and antigen processing targets ensures reliable sourcing support for your diagnostic and research needs.