Anti-PSMB7 Polyclonal Antibody for WB, IF/ICC, ELISA - Q99436

Core Product Specifications and Parameters

Biological Background: PSMB7 Function and Localization

• PSMB7 (proteasome subunit beta type-7), also known as macropain chain Z or multicatalytic endopeptidase complex chain Z, is a core component of the 20S proteasome complex (UniProt Q99436).
• It participates in ATP-dependent degradation of ubiquitinated proteins within the 26S proteasome, maintaining protein homeostasis by removing misfolded, damaged, or temporally unneeded proteins.
• The subunit mediates ubiquitin-independent proteolysis when the 20S core associates with regulatory particles like PA200 or PA28, important for processes such as spermatogenesis and MHC class I antigen presentation.
• PSMB7 exhibits trypsin-like catalytic activity, cleaving peptide bonds after basic residues.
• The protein localizes to both the cytoplasm and nucleus.
• Expression is low in normal colonic mucosa but significantly upregulated in colorectal cancer tissues, implicating PSMB7 in tumorigenesis.
• The protein is synthesized as a zymogen precursor that undergoes autocatalytic processing to generate the mature active subunit.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fragment covering amino acids 44–200, which includes part of the mature subunit; the antibody may recognize both precursor (≈30 kDa) and mature forms.
• For Western blot, a single band near 30 kDa is expected; consider validating specificity using siRNA knockdown or knockout cell lysates.
• For immunofluorescence/ICC, optimize fixation and permeabilization conditions; nuclear and cytoplasmic staining patterns may be observed.
• In ELISA applications, verify the antibody's performance as a capture or detection reagent through standard pairing experiments.
• Cross-reactivity with mouse and rat PSMB7 allows multi-species studies; however, always confirm reactivity in your experimental system.

CamelBio: Your One-Stop Sourcing Bridge

As your one-stop IVD raw-material partner, CamelBio supplies this anti-PSMB7 polyclonal antibody along with validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing—supporting proteasome research and cancer assay development. From concept to clinic, CamelBio bridges your sourcing gaps with reliable, cost-effective raw materials and technical expertise.