Anti-PSMB6 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P28072

Core Product Specifications and Parameters

Biological Background: PSMB6 Function and Localization

• PSMB6, also known as LMPY, DELTA, or proteasome subunit beta-1, is a beta-type subunit of the 20S core proteasome complex, a ~700 kDa barrel-shaped structure responsible for degrading most intracellular proteins.
• Within the 20S core, PSMB6 displays peptidylglutamyl‑hydrolizing (postacidic or caspase‑like) activity, cleaving peptide bonds directly after acidic residues.
• By associating with two 19S regulatory particles, PSMB6 forms the 26S proteasome, which mediates ATP‑dependent degradation of ubiquitinated proteins, maintaining protein homeostasis by eliminating misfolded, damaged, or no‑longer‑required proteins.
• Together with the PA200 or PA28 activators, the 20S proteasome (including PSMB6) performs ubiquitin‑independent proteolysis, critical for processes such as spermatogenesis (via 20S‑PA200) and generation of a subset of MHC class I‑presented antigenic peptides (via 20S‑PA28).
• PSMB6 is primarily localized in the cytoplasm and nucleus, consistent with the broad intracellular distribution of proteasomes.
• The protein undergoes post‑translational modifications including acetylation and phosphorylation, and is synthesized as a zymogen with a propeptide removed for activation.
• PSMB6 is widely expressed and belongs to the peptidase T1B family of threonine proteases, essential for regulated proteolysis across virtually all cell types.

Experimental Guidance and Technical Tips

• The immunogen encompasses amino acids 35‑239 of human PSMB6, corresponding to the mature subunit after propeptide cleavage; the antibody may therefore recognize both precursor and mature forms in some samples. Consider including appropriate protease inhibitors during lysis to preserve the zymogen form if detection is desired.
• For Western blotting, a band at approximately 25 kDa is expected; validate in the relevant sample system and normalize loading with a suitable housekeeping protein.
• For immunohistochemistry (IHC‑P) and immunofluorescence (IF/ICC), standard antigen retrieval and blocking protocols are recommended. Optimize primary antibody dilution and incubation conditions for each tissue or cell type.
• Cross‑reactivity with human, mouse, and rat samples has been reported; however, consider confirming specificity with appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a professional IVD raw‑material partner, providing diagnostic manufacturers and research laboratories with validated antibodies, bulk ancillary reagents, and tailored supply solutions—from early‑stage development to clinical application. We support proteasome‑related and protein‑degradation research with carefully selected anti‑PSMB6 reagents and can assist in sourcing rare‑target raw materials to streamline your workflow.