Anti-PSMB5 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P28074

Core Product Specifications and Parameters

Biological Background: PSMB5 Function and Localization

• PSMB5 encodes the proteasome subunit beta type-5, also known as macropain epsilon chain, proteasome subunit MB1, or proteasome subunit X.
• It is a component of the 20S core proteasome complex responsible for the proteolytic degradation of most intracellular proteins.
• The 20S proteasome can associate with 19S regulatory particles to form the 26S proteasome, mediating ATP-dependent degradation of ubiquitinated proteins, crucial for protein homeostasis.
• PSMB5 specifically displays chymotrypsin-like activity within the 20S core complex.
• The 20S proteasome also mediates ubiquitin-independent degradation when associated with PA200 or PA28, involved in spermatogenesis and MHC class I antigen processing.
• The protein localizes to both the cytoplasm and nucleus.
• Post-translational processing: The protein is synthesized as a zymogen precursor that is cleaved during proteasome assembly.
• Additional features include alternative splicing and involvement in host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the full-length protein (aa 1-263), so the antibody is expected to recognize the native and denatured form under various assay conditions.
• For Western blot (WB), a band around 28kDa is expected; validate in your specific sample and loading controls.
• In immunoprecipitation (IP), consider using appropriate negative controls to confirm specificity.
• For IHC-P and IF/ICC, tissue and cell fixation/permeabilization may affect antigen accessibility; optimize conditions.
• The antibody cross-reacts with mouse and rat, but sequence homology should be confirmed for other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive sourcing solutions for IVD raw materials, supporting diagnostic manufacturers and research laboratories from concept to clinic. This anti-PSMB5 antibody is part of our extensive catalog for proteasome and cancer research, and we also supply validated antibody pairs, recombinant proteins, and bulk ancillary reagents. For rare or custom targets, our global network enables efficient procurement to accelerate your assay development.