Anti-PSMB4 Rabbit Polyclonal Antibody for WB and ELISA - P28070

Core Product Specifications and Parameters

Biological Background: PSMB4 Function and Localization

• PSMB4 encodes the proteasome subunit beta type-4, a non-catalytic component of the 20S core proteasome complex, essential for intracellular protein degradation.
• It participates in the ATP-dependent degradation of ubiquitinated proteins as part of the 26S proteasome, which removes misfolded or regulatory proteins to maintain protein homeostasis.
• PSMB4 also mediates ubiquitin-independent protein degradation via association with PA200 or PA28 regulatory particles, playing roles in spermatogenesis and MHC class I antigen presentation.
• A SMAD1/OAZ1/PSMB4 complex promotes the degradation of the transcriptional repressor SNIP1, linking the proteasome to signaling pathways.
• The protein localizes to both the cytoplasm and nucleus, consistent with its broad role in protein turnover.
• Post-translational modifications include acetylation and phosphorylation, as identified by proteomics and direct sequencing.
• Mutations in PSMB4 are associated with proteasome-associated autoinflammatory syndrome 3 (PRAAS3), highlighting its clinical relevance.

Experimental Guidance and Technical Tips

• For Western blot, expect a band around 29 kDa (predicted molecular weight 29.2 kDa). Validate antibody specificity using PSMB4 knockout lysates or peptide competition assays.
• The immunogen corresponds to full-length human PSMB4 (aa 1–264), suggesting recognition of the native protein; confirm performance in relevant biological samples.
• Cross-reactivity with mouse and rat is stated; however, always verify reactivity in your specific model system before large-scale experiments.
• For ELISA, consider direct antigen coating or sandwich formats; optimize antibody dilution and detection conditions empirically.
• When probing endogenous levels, account for potential variations in expression across tissues and cell lines by including appropriate loading controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one-stop procurement channel for IVD raw materials, including this anti-PSMB4 polyclonal antibody for studying the ubiquitin-proteasome system. Beyond antibodies, our portfolio encompasses validated antibody pairs, optimized monoclonal and polyclonal products, ancillary reagents, and rare-target sourcing to support your assays from concept to clinic. Let CamelBio be your streamlined bridge to high-quality, publication-ready reagents.