Anti-PSMB3 Polyclonal Antibody for WB, IHC-P, ELISA - P49720

Core Product Specifications and Parameters

Biological Background: PSMB3 Function and Localization

• PSMB3 is a non-catalytic subunit of the 20S core proteasome complex, essential for intracellular protein degradation.
• Alternate names include Proteasome chain 13, Proteasome component C10-II, and Proteasome theta chain.
• The 20S proteasome associates with 19S regulatory particles to form the 26S proteasome, mediating ATP-dependent degradation of ubiquitinated proteins.
• With PA200 or PA28 regulator, the 20S proteasome facilitates ubiquitin-independent proteolysis, important for spermatogenesis (20S-PA200) and MHC class I antigen presentation (20S-PA28).
• PSMB3 localizes to both the cytoplasm and the nucleus, consistent with its role in protein turnover throughout the cell.
• Post-translational modifications include acetylation; the protein is a target of proteasome functionality.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein covering amino acids 1-205 of human PSMB3. Consider verifying antibody specificity by peptide competition or knockdown validation.
• For Western blot, a band ~23kDa is expected. Use whole cell lysates from human, mouse, or rat cells as positive controls.
• For immunohistochemistry (paraffin sections), optimize antigen retrieval; PSMB3 expression is widespread, so test appropriate positive tissue.
• For ELISA, the recombinant immunogen may serve as a coating antigen to assess antibody reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw material supply for proteasome research, including validated antibody pairs and custom polyclonal antibodies. This anti-PSMB3 product exemplifies our commitment to supporting diagnostic manufacturers with reliable reagents. We also offer bulk ancillary reagents and facilitate sourcing of rare-target antibodies to streamline your assay development.