Anti-PSMB2 Polyclonal Antibody for WB, IHC-P, IP, ELISA - P49721

Core Product Specifications and Parameters

Biological Background: PSMB2 Function and Localization

• PSMB2 (proteasome subunit beta type-2), also known as macropain subunit C7-I or proteasome component C7-I, is a non-catalytic subunit of the 20S proteasome core particle.
• As part of the 26S proteasome, PSMB2 contributes to the ATP-dependent degradation of ubiquitinated proteins, essential for protein homeostasis and the removal of misfolded or regulatory proteins.
• The 20S proteasome can also perform ubiquitin-independent proteolysis when associated with regulatory particles PA200 or PA28, which is important for spermatogenesis and the generation of MHC class I peptides.
• PSMB2 localizes to both the cytoplasm and nucleus, consistent with the proteasome's broad role in cellular protein turnover.
• The protein is subject to acetylation and is implicated in host-virus interactions, as noted by UniProt keywords.
• With a molecular weight of approximately 23 kDa, PSMB2 is encoded by the PSMB2 gene (Gene ID: 5690).

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 1-201 of human PSMB2, a region conserved in mouse and rat, supporting its cross-species reactivity for WB and IHC-P.
• For Western blotting, a specific band around 23 kDa is expected; consider using protease inhibitors and optimizing protein loading to avoid degradation.
• For immunohistochemistry (IHC-P), antigen retrieval methods and antibody titration should be optimized for the specific tissue type and fixation conditions.
• This polyclonal antibody is suitable for immunoprecipitation; validate using appropriate positive and negative controls, and consider cross-linking for co-IP experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing partner for IVD raw materials, supporting diagnostic developers from early research through clinical application. For proteasome-related targets such as PSMB2, we supply validated polyclonal antibodies, optimized recombinant proteins, and a range of ancillary reagents. Our technical consulting and supply chain capabilities help accelerate your assay development and manufacturing.