Anti-PSMB2 Polyclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P49721

Core Product Specifications and Parameters

Biological Background: PSMB2 Function and Localization

• Proteasome subunit beta type-2 (PSMB2) is a non-catalytic component of the 20S core proteasome complex, which is responsible for the proteolytic degradation of most intracellular proteins.
• As part of the 20S proteasome, PSMB2 contributes to proteasome structure; when associated with two 19S regulatory particles, it forms the 26S proteasome that degrades ubiquitinated proteins in an ATP-dependent manner.
• The 26S proteasome is critical for protein homeostasis, removing misfolded or damaged proteins and regulating protein levels.
• The 20S proteasome, in complex with PA200 or PA28, mediates ubiquitin-independent protein degradation, playing roles in spermatogenesis and generation of MHC class I-presented antigenic peptides.
• PSMB2 localizes to both the cytoplasm and nucleus.
• Post-translational modifications include acetylation, which may regulate proteasome function.
• PSMB2 is involved in host-virus interactions, though specific mechanisms may require further study.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (amino acids 1-201), which may provide broad recognition of PSMB2; consider using peptide blocking to confirm specificity.
• For Western blotting, PSMB2 has a predicted molecular weight of ~23 kDa (observed around 23 kDa); use appropriate gel percentage for detection.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), both cytoplasmic and nuclear staining may be observed due to its subcellular localization.
• For immunoprecipitation (IP), validate efficiency in relevant cell lines expressing PSMB2.
• ELISA applications may require optimizing coating antigen and antibody concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. This anti-PSMB2 antibody supports proteasome and protein degradation research, and we can supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your development pipeline.