Anti-PSMB10 Rabbit Monoclonal Antibody for WB, ELISA - P40306

Core Product Specifications and Parameters

Biological Background: PSMB10 Function and Localization

• PSMB10 encodes the proteasome subunit beta type-10, also known as LMP10, MECL1, or beta-2i, a catalytic subunit of the 20S immunoproteasome.
• As a component of the immunoproteasome, it specializes in generating peptide ligands for MHC class I molecules, playing a central role in antigen processing.
• The proteasome cleaves peptides after Arg, Phe, Tyr, Leu, and Glu residues at neutral to slightly basic pH, with ATP-dependent proteolytic activity.
• Subcellularly, PSMB10 localizes to both the cytoplasm and the nucleus, consistent with proteasome distribution.
• The protein undergoes post-translational modifications including acetylation and phosphorylation (evidenced by proteomics identification).
• The alias PRAAS5 links PSMB10 to proteasome-associated autoinflammatory syndromes; disease variants have been reported.
• PSMB10 is expressed as a zymogen precursor that undergoes autocatalytic processing to the mature threonine protease.
• Keywords highlight its involvement in host-virus interactions and availability of 3D structural data.

Experimental Guidance and Technical Tips

• For Western blotting, use reducing conditions and validate with positive lysates from human, mouse, or rat cells; the predicted band size is ~29 kDa (UniProt: 28936 Da).
• Endogenous PSMB10 may show varying expression across cell types; consider using interferon-gamma-stimulated samples to upregulate immunoproteasome subunits.
• For ELISA, optimize coating buffer and antibody dilution; the antibody may recognize both native and denatured forms based on the full-length recombinant immunogen.
• Perform sequence alignment of human, mouse, and rat PSMB10 to confirm epitope conservation if species cross-reactivity data is required beyond western blot.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement, offering a single contact point for validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Whether you need this PSMB10 antibody for proteasome-related immunoassays or have rare target sourcing needs, our team supports diagnostic manufacturers and research institutes from concept to clinic. Let us bridge your supply gaps with reliable, high-quality biological materials.