Anti-PSMB1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P20618

Core Product Specifications and Parameters

Biological Background: Proteasome subunit beta type-1 Function and Localization

• Proteasome subunit beta type-1 (PSMB1) is a non-catalytic component of the 20S core proteasome complex, essential for the proteolytic degradation of most intracellular proteins.
• As part of the 26S proteasome, it participates in ATP-dependent degradation of ubiquitinated proteins, crucial for protein homeostasis and removal of misfolded or damaged proteins.
• In the 20S proteasome, it also mediates ubiquitin-independent protein degradation, required for processes like spermatogenesis and MHC class I antigen presentation.
• PSMB1 localizes to both the cytoplasm and nucleus, reflecting its broad role in cellular proteolysis.
• Post-translational modifications include acetylation and phosphorylation, as indicated by UniProt keywords.
• Genetic associations include deafness and intellectual disability, and the protein is involved in host-virus interactions.
• The molecular weight of PSMB1 is approximately 26.5 kDa, consistent with the observed protein weight around 26 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 29-241 of human PSMB1, suggesting the antibody recognizes the mature protein; consider validating in the relevant sample system.
• For Western blot, expect a band near 26 kDa; optimize loading and antibody dilution for clear detection.
• In immunofluorescence/immunocytochemistry, fixation and permeabilization conditions may require optimization for specific cell types.
• For ELISA, the antibody can be used for detection; consider testing with recombinant protein or cell lysates as coating antigen.
• Cross-reactivity with mouse and rat has been reported, but verify specificity in your experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material sourcing for proteasome research, offering validated antibody pairs, optimized polyclonal antibodies like this anti-PSMB1, and bulk ancillary reagents. Backed by comprehensive technical support and rare-target procurement capabilities, we accelerate your assay development from concept to clinic.